Gene deletion - Constitutive knock out mouse |
|
Gene deletion is based on the inactivation at the genetic level of a gene of interest.
This can be achieved through random mutation (gene trap approach, chemical mutagenesis …) or targeted insertion (homologous recombination). genOway has developed an offer based on the use of a homologous recombination vector and embryonic stem cells. Homologous recombination provides the customer with the best adapted model and the lowest risk.
|
|
The constitutive "knock out" (knockout) approach has the following characteristics.
|
|
| Advantages |
Drawbacks |
|
Total inactivation of the gene in any cell
|
Phenotypes can be complex since all organs are affected
|
|
|
The constitutive "knock out" (knockout) model provides a broad overview of gene function. |
|
genOway has developed an innovative technology " Safe Knock-out™" enabling the generation of constitutive and conditional knock-out lines starting from the same targeted ES cell clones. This reduces the risk associated with constitutive knock-outs (lethality, complex phenotype…) by allowing to switch to conditional models if necessary. This approach avoids time delay and facilitates model up-grading. |
 |
|
|
The offer genOway has developed can include the following steps, but the service is flexible (can be limited to part of this offer and/or adapted to customer requirements) : |
|
| Step 1 : |
Subcloning and characterization of the locus of interest |
| Step 2 : |
Knock out / knockout vector construction (targeting vector) |
| Step 3 : |
Homologous recombination in Embryonic Stem cells (ES cell) |
| Step 4 : |
Blastocyst injection and chimera generation |
| Step 5 : |
Breeding to the F1 and F2 generation |
|
|
The development of the first cloned rat reported by genOway in Science ( Zhou et al. 2003) paves the way for the development of knock out rat models and allows new opportunities for research areas focused on rat models. This technology is not yet available as a service. Only research partnerships may be established. |
 | |
|
|
Service offer
Ready-to-use models