Gene deletion - Conditional knock out mouse |
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This strategy, conditional knock out, is based on a tissue-specific inactivation of the gene of interest.
This can be achieved by means of a recombinase. A recombinase is an enzyme that deletes the DNA fragment located between the two recombinase-specific sites. A mouse bearing the recombinase-specific sites (introduced by homologous recombination in Embryonic Stem cells) is bred with a mouse expressing the recombinase (generated by homologous recombination or transgenesis). The tissue-specific expression of the recombinase allows the inactivation of the gene of interest only in the tissue where the recombinase is expressed. The final performance of the model is strongly dependent on the quality of the recombinase expressing mouse.
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| Advantages |
Drawbacks |
Inactivation is restricted to specific tissues
Several tissue-specific models can be made in parallel
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Efficiency of the excision
Recombinase validated system
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The conditional "knock out" (knockout) strategy provides a research model of high potential. |
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To create a conditional "knock out" (knockout) model, the genetically modified model generated should be bred with a mouse line expressing the recombinase in the tissue of interest. |
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genOway has developed an innovative technology " Safe Knock-out™" enabling the generation of constitutive and conditional knock-out lines starting from the same targeted ES cell clones. This reduces the risk associated with constitutive knock-outs (lethality, complex phenotype…) by allowing to switch to conditional models if necessary. This approach avoids time delay and facilitates model up-grading. |
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The offer genOway has developed can include the following steps, but the service is flexible (can be limited to part of this offer and/or adapted to customer requirements) |
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| Step 1 : |
Subcloning and characterization of the locus of interest |
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&Knock out / knockout vector construction (targeting vector) |
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Homologous recombination in Embryonic Stem cells (ES cell) |
| Step 4 : |
Blastocyst injection and chimera generation |
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Breeding to the F1 and F2 generation |
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The development of the first cloned rat reported by genOway in Science ( Zhou et al. 2003) paves the way for the development of conditional knock out rat models and allows new opportunities for research areas focused on rat models. This technology is not yet available as a service. Only research partnerships may be established. |
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Service offer
Ready-to-use models