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Service offer

Gene overexpression

Humanization

Gene deletion

Gene Knock down (RNAi)

Gene Knock down (RNAi)

RNA interference (RNAi) is a mechanism in which double-stranded RNAs inhibits corresponding gene expression by inducing degradation of its mRNA.
RNAi approaches have become state-of-the-art methods for in vitro target validation.

Invitrogen Corporation and genOway are working together to provide
a complete RNAi solutions platform for the in vivo use of shRNA to researchers worldwide
Click here for more information

genOway has developed a panel of technologies that secures in vivo gene Knock-down.

Random insertion of the shRNA construct into the mouse genome

genOway developed the "Safe RNAi Transgenesis™" technology proven efficient for in vivo RNAi transgenesis:

	shRNA copy number is controlled
	Access to high numbers of founders to avoid shRNA position effects
	Short development time (similar to DNA microinjection)

The offer genOway has developed can include the following steps, but the service is flexible (can be limited to part of this offer and/or adapted to customer requirements)

Step 1 :	Construction of the shRNA expression vector
Step 2 :	Random integration of the shRNA expression vector
Step 3 :	Blastocyst injection and chimera generation
Step 4 :	Breeding to the F1 and F2 generation

Targeted insertion of the shRNA construct into the mouse genome

The "Safe in vivo RNAi™" technology enables a targeted insertion (knock-in) of the shRNA construct and secures its expression:

	Access to ready-to-use targeting vectors suitable for spatio-temporal control of shRNA expression
	For in vivo validation of shRNA and relevant comparative studies
	The target gene can be subsequently removed to study shRNA off-target effects.

The offer genOway has developed can include the following steps, but the service is flexible (can be limited to part of this offer and/or adapted to customer requirements)

Step 1 :	Insertion of the shRNA of interest into genOway ready-to-use knock-in vector
Step 2 :	Homologous recombination in Embryonic Stem cells (ES Cell)
Step 3 :	Blastocyst injection and chimera generation
Step 4 :	Breeding to the F1 and F2 generation

For more information,
please e-mail us at info@genoway.com or visit our information request page

Transgenics (rat and mouse gene modification): knockout mouse (KO mouse), knock in mice (KI mice) and transgenic mouse
genOway, service provider in transgenesis for customized genetically modified mice and rats: gene overexpression, gene knockdown, gene knockout, etc.
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