Humanization |
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Humanization of animal models has proved hugely promising tools to decipher physiological processes and increasing the predictability of animal model. Based on Knock-in approach (targeted insertion of the transgene in a selected locus), a humanization consists in the replacement of the murine gene by its human counter part. |
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Drawbacks |
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Specific integration site |
Molecular biology work can be more complex and longer
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Totally controlled transgene genetic environment
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The human gene is controlled by the endogenous promoter and regulatory elements
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Only one transgenic line is necessary
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Study of human gene function by mouse gene humanization: genOway has patented several innovative strategies based on the design of evolving animal models to anticipate subsequent experimental steps. This approaches are time-saving and cost effective. |
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The offer genOway has developed can include the following steps, but the service is flexible (can be limited to part of this offer and/or adapted to customer requirements) : |
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| Step 1 : |
Cloning and characterization of the murine gene of interest |
| Step 2 : |
Cloning and characterization of the human cDNA/gene of interest |
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Knock-in / Knockin vector construction (targeting vector) |
| Step 4 : |
Homologous recombination in Embryonic Stem cells (ES Cell) |
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Blastocyst injection and chimera generation |
| Step 6 : |
Breeding to the F1 and F2 generation |
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Service offer
Ready-to-use models