Constitutive KO

genOway provides a one-stop solution for the design, creation and analysis of your custom knockout mouse model.

Step 1: Scientific evaluation

genOway has a dedicated team of scientists and bioinformatics experts who are able to perform thorough analyses of your project feasibility including:

• Defining the model's objectives
• Determining the characteristics of the target gene/locus
• Analyzing data culled from the literature

Step 2: Design of genetically modified model

We evaluate the possible gene targeting options based on the results of the above scientific evaluation and present the design to the principal investigator with a clear assessment of model features, risks and timelines.

• Our process guides you toward the best choice for your model design.
• We provide free and non-binding access to this expertise.

Step 3: Production

genOway has an extensive background in genetically modified mouse technology and will provide you with the highest quality product possible. Four mouse knockout production options are available. All options include the same dedicated customer support.

Option 1:  Full service

Your project is managed from initial conception to final realization by our in-house transgenesis experts. This level of service includes:

• Model design, starting from a gene accession number
• Model creation up to validated heterozygous/homozygous animals
• Cohort production
• Phenotyping  (i.e. physiological validation of the model along with a comprehensive array of tests)

Option 2:  Standard service

genOway manages your project from initial conception (starting from a gene accession number) to the delivery of heterozygous animals.

Option 3:  Partial production service

Exit the model creating process at any step of your choosing (such as vector construction or recombined ES cell line creation).

Option 4:  Animal production service

genOway manages your model creation project, starting from existing targeting vectors or ES cell clones. These vectors or clones are either developed in your lab or available via the International KO Mouse Consortium (IKMC – EUCOMM, KOMP, NorCOMM).

Milestones	Full	Standard	Partial production	Animal production
Project evaluation	Bioinformatics analysis, bibliography search, project feasibility assessment			Analysis of the selected strategy, risk assessment
Model design and targeting vector construction	x	x	x
Recombined ES cells	x	x	optional	optional
Blastocyst injection	x	x	optional	x
Heterozygous animals	x	x		x
Homozygous animals	x	optional		optional
Cohort production	x	optional		optional
Phenotyping	x	optional		optional