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 Knock-in (targeted insertion) approach overcomes DNA micro-injection limits |
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More than 450 publications (PubMed) have demonstrated that targeted insertion provides:
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A more predictable expression pattern than does random integration (transgenic) |
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A unique and fully characterised integration site enabling in vivo analysis on a single mouse line. |
But each model requires a new targeting vector (2 to 6 months) and suffers from uncertainty of gene targeting.
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Intellectual Property
Knock-in is a general term for models expressing a transgene targeted in a
selected locus. Several technologies exist to create such models. These
technologies use techniques (alone or in combination) such as homologous
recombination, recombinases, insertion shuttles, etc.
genOway can implement technologies that are free of use for customers and in
particular that are not depending from the Pasteur Institute patent family
(abusively called Homologous recombination technology by Cellectis).
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| HPRT locus enables ubiquituous and tissue-specific expression |
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More than 50 publications (PubMed) have demonstrated that HPRT locus enables ubiquitous and tissue-specific expression.
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"Quick Knock-in"™ benefits: fast & safe |
| Fast |
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Free access to a library of ready-to-use targeting vectors |
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Free access to cassettes for constitutive, tissue specific and inducible expression |
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Free access to validated 5’ and 3’ screening for both PCR and Southern Blot |
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| Safe |
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Guaranteed Homologous Recombination (4% to 8%) through an optimized targeting vector |
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Generation of targeted ES cell is guaranteed |
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Recombinase Mediated Cassette Exchange strategy (RMCE) |
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Generation of chimeras & heterozygotes is guaranteed |
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| "Quick Knock-in"™ is cost-effective: |
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The targeting vector is free of charge |
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The 5’ and 3’ screening set up for both PCR and Southern blot is free of charge |
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| "Quick Knock-in"™ is time-effective: |
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Ready-to-use targeting vectors & molecular screening |
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High and secured homologous recombination frequency |
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Charles River Laboratories delivers animals to your breeding unit.
No quarantine or derivation is required |
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| Starting point: customer provides his trangene |
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| Vector construction |
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3 |
| Homologous recombination |
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6 |
| Screening PCR Southern Blot 5’ & 3’ |
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4 |
| Blastocyst injection |
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2 |
| Adult chimeras (germline guaranteed) |
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7 |
| + 11 weeks per mouse generation |
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22 weeks |
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Service offer
Technology platform
