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 Knock-in (targeted insertion) approach overcomes DNA micro-injection limits |
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More than 450 publications (PubMed) have demonstrated that targeted insertion provides:
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A more predictable expression pattern than does random integration (transgenic) |
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A unique and fully characterised integration site enabling in vivo analysis on a single mouse line. |
But each model requires a new targeting vector (2 to 6 months) and suffers from uncertainty of gene targeting.
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| HPRT locus enables ubiquituous and tissue-specific expression |
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More than 50 publications (PubMed) have demonstrated that HPRT locus enables ubiquitous and tissue-specific expression.
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"Quick Knock-in"™ benefits: fast & safe |
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Free access to a library of ready-to-use targeting vectors |
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Free access to cassettes for constitutive, tissue specific and inducible expression |
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Free access to validated 5’ and 3’ screening for both PCR and Southern Blot |
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| Safe |
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Guaranteed Homologous Recombination (4% to 8%) through an optimized targeting vector |
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Generation of targeted ES cell is guaranteed |
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Recombinase Mediated Cassette Exchange strategy (RMCE) |
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Generation of chimeras & heterozygotes is guaranteed |
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| "Quick Knock-in"™ is cost-effective: |
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The targeting vector is free of charge |
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The 5’ and 3’ screening set up for both PCR and Southern blot is free of charge |
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| "Quick Knock-in"™ is time-effective: |
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Ready-to-use targeting vectors & molecular screening |
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High and secured homologous recombination frequency |
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Charles River Laboratories delivers animals to your breeding unit.
No quarantine or derivation is required |
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| Starting point: customer provides his trangene |
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| Vector construction |
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3 |
| Homologous recombination |
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6 |
| Screening PCR Southern Blot 5’ & 3’ |
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4 |
| Blastocyst injection |
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2 |
| Adult chimeras (germline guaranteed) |
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7 |
| + 11 weeks per mouse generation |
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22 weeks |
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Service offer
Technology platform
