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This could be overcome by analysing a high number of founders allowing to bypass the position effect and to have statistical results correlated to transgene expression.

The number of founders generated by DNA pronuclear microinjection can not be predicted, since the efficiency of this technology ranks from 0% to 10%, based on literatures and personal communication.

Red curve in the figure presented in the central panel represents the average number of founders generated per experiment using classical DNA Tranegenesis.
The average number of founders obtained by using this technology ranks from 0 and 4. 60 to 70% of experiments resulted in a low number of founders (<3), therefore requiring additional experiments to achieve a sufficient number of founders, for relevant in vivo analysis.

Moreover, this unpredictable information is known at the very end of the project, when the animals are genotyped.
This strongly impacts project development timelines and costs.

On average, this novel technology allows the generation of 10 to 30 lines, even if in general scientists first focus on 4 to 7 lines displaying different integration sites and different copy numbers for a first analysis.

Green curve in the figure presented in the central panel represent the average number of founders generated per experiment using : "Safe DNA Transgenesis™". Based on our experience using "Safe DNA Transgenesis™", the average number of lines that can be developed in one experiment ranks from 10 to 30.

This approach allows generation of high number of founders in a single shot experiment, therefore securing the project issue (sufficient number of founders for relevant in vivo studies) and allowing shorter development times and lower development costs.