Reliable & Innovative Solutions for Transgenesis| home | contact us |

Technology platform
 
   Service offer
   Ready-to-use models
   Technology platform
      Safe DNA Transgenesis™
      Quick Knock-in™
      Safe RNAi Transgenesis™
      Safe in vivo RNAi™
      Genetically modified Rats
      Rat cloning
   Our customers
   R&D pipeline
   Corporate overview
   Investor's room
   Careers
   News room
   Contact us

"Safe in vivo RNAi"™ technologies: Guaranteed Heterozygous Animals

RNA interference (RNAi) has become the state-of-the-art method for in vitro target validation.
genOway is pleased to introduce its new Knock-down technologies.

"Safe in vivo RNAi"™ Technologies:
Targeted insertion (Knock-in) of the shRNA into the HPRT locus
Efficient: single copy of shRNA targeted into the HPRT locus
A single copy of the shRNA is targeted into the HPRT locus
The HPRT locus enables ubiquitous, tissue-specific and inducible expression of the shRNA construct
Only one single transgenic line is required
In vivo comparison of shRNA functions
More relevant than viral-mediated delivery:
Targeted insertion of the shRNA versus random integration
One single transgenic mouse line versus 3 to 5 transgenic lines
No segregation issue
Same development time
Fast: Ready-to-use tools provided by the "Safe in vivo RNAi"™ technologies
Free access to ready-to-use and validated targeting vectors for shRNA expression
Free access to validated 5’ and 3’ screening for both PCR and Southern Blot
Safe: Guarantees provided by the "Safe in vivo RNAi"™ technologies
Generation of targeted ES cells is guaranteed
Generation of chimeras and heterozygotes is guaranteed

Applications
"RNAi Quick Knock-in"™:
shRNA Knock-in mouse guaranteed in 22 weeks
Targeted insertion of the shRNA into the HPRT locus: safe & fast Knock-down approach
Secured shRNA expression
One single line for in vivo studies
In vivo comparison of shRNA functions

"Inducible in vivo RNAi"™:
shRNA Knock-in mouse guaranteed in 26 weeks
Temporal regulation of the shRNA expression enabling time-controlled gene Knock-down
Applications
Loss of function of essential genes
Target validation in physiopathological conditions
Prof. Thomas Rosenquist (Director Transgenic Mouse Facility, SUNY-Stony Brook University, New York).
"Germline transmission of RNAi in mice" Nat. Struct. Biol. 2003, 10(3):
"Low copy number seems to be key to getting RNAi working in mice.
The target gene knockdown is stable through F2 generation mice and we have tested tissues from mice up to 6 months old".
In 2 years, genOway has become the leading company for in vivo RNAi rodent models
2002 Development of the "Safe RNAi Transgenesis"™ technology, Knock-down mouse model based on random integration
2003 Expediting Target Identification and Validation through RNAi meeting (London): "Essential Requirements for Successful Use of RNAi in Transgenic Mice"
2004 Launch "RNAi Quick Knock-in"™ based on targeted insertion of the shRNA
For more information,
please e-mail us at info@genoway.com or visit our information request page
Transgenics (rat and mouse gene modification): knockout mouse (KO mouse), knock in mice (KI mice) and transgenic mouse
genOway, service provider in transgenesis for customized genetically modified mice and rats: gene overexpression, gene knockdown, gene knockout, etc.
| Site map | Legal Notice | © genOway 2006 |