Gene overexpression - Random integration : transgenic mouse and rat |
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This approach is based on random integration of the transgene in the genome.This technique is available for both mice and rats. |
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| Advantages |
Drawbacks |
Simple molecular biology work
Short development time
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Unknown integration site
Variable copy number
Requirement of several independent transgenic lines
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The final characteristics (expression pattern & level of expression) and relevance of the transgenic mouse or transgenic rat obtained are unpredictable, more over all the research work must be performed on several independent lines. |
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Pronuclear microinjection
This service is adapted for fast and secured transgenic rodent model creation in a variety of strains including C57BL/6, OF1 and B6DBA2 for the mouse and Sprague Dawley for the rat.
Please visit our " Genetically modified Rat" page for more information on the service available for the rat model.
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ES cell based gene overexpression
genOway has developed innovative and reliable technologies that reduce the risks associated with the development of transgenic mouse line by DNA pronuclear random integration.
Please visit our " Safe DNA transgenesis™" and " Safe RNAi transgenesis™" pages.
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Large fragment transgenesis
genOway proposes the "Safe Large DNA transgenesis™" technology., an innovative service reducing the risks associated with large DNA fragment transgenesis (BAC, YAC, PAC,...). |
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The offer genOway has developed can include the following steps, but the service is flexible (can be limited to part of this offer and/or adapted to customer requirements) : |
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| Step 1 : |
Cloning of the cDNA of interest |
| Step 2 : |
Cloning of the promoter of interest |
| Step 3 : |
Construction of the expression vector |
| Step 4 : |
Random integration of DNA : generation of transgenic mouse and transgenic rat |
| Step 5 : |
Breeding to the F1 and F2 generation |
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Custom model production
Transgenic mouse lines