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Gene invalidation in mouse intestine
The Villin-Cre expressing mouse line is now available through genOway
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Cre recombinase expression pattern and activity in the Villin-Cre mouse:
- Expressed in 100% of epithelial cells of the small and large intestines
- Broadly expressed in the adult in every cell of the intestinal epithelium on both the vertical axis (crypt to villus tip) and the horizontal axis (duodenum through colon) of the intestine
Figure 1: ß-galactosidase expression due to Cre recombinase activity in 4-week-old mice produced by mating homozygous Rosa26 conditional reporter mice to hemizygous transgenic founders carrying the 12.4kbVilCre construct.
A. 12.4kbVilCre transgenic intestines exhibit continuous expression of b-galactosidase:
d, duodenum in the pyloric region, showing the stomach/duodenum border, where a sharp boundary of expression is seen, with no expression in the stomach (arrow)
J, jejunum - I, ileum - c, colon
B. Cre was expressed continuously as evidenced by non-variegated b-galactosidase staining; levels of Cre were sufficient to allow recombination in every cell.
C. Mosaic expression indicated by wholly positive crypts surrounded by wholly negative ones.
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Villin-Cre mouse model description:
A 12.4-kb promoter fragment from the villin gene drives the expression of Cre recombinase in the Villin-Cre mouse. When crossed with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in intestine-specific deletion of the target gene.
Mouse line access
- Fully licensed mouse line: no further negotiation with the mouse line owner
- Genetic background is C57BL/6 ( N5+ backcrossed)
- Mice delivered are SOPF /VAF certified by Charles River Laboratories
- Worldwide delivery using the logistic network of Charles River Laboratories
- Madison et al., J.Bio.Ch., 277, 33275-33283 (2002)
- Braunstein et al., Dev.Dyn. 224, 90-102 (2002)
- Maunoury et al., Development, 115, 717-728 (1992)
- Mori-Akiyama et al., Gastroenterology. Aug;133(2):710-2 (2007)
- Yoshida Y. et al., Gastroenterology. Apr;132(4):1420-31 (2007)
- Kucherlapati MH et al., Cancer Res. Apr 1;66(7):3576-83 (2006)
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Service offer
Ready-to-use models
