Preclinical Humanized OX40 Mouse Model

Humanized Immune Checkpoint Mouse Models

Design of the hOX40 mouse

The humanized OX40 mouse model (hOX40) is developed by Knockin at the mouse OX40 locus, and expresses a fully humanized OX40 with a human extracellular, transmembrane and intracellular domain.

Applications in immuno-oncology

The hOX40 mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint OX40 in fully immunocompetent mice.

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hOX40 features

Physiological regulation and expression pattern of the human OX40
Preservation of the target-ligand interaction
Fully functional mouse immune system
Lack of expression of the murine target gene, thus avoiding cross-reactivity

hOX40 validation*

hOX40 ICP model validation 1

hOX40 expression pattern recapitulates mOX40

hOX40 and mOX40 expression on splenocytes activated with αCD3/αCD28 in presence of mIL-2. Kinetic of hOX40 and mOX40 expression on Treg, conventional CD4 and CD8 T cells. Results are expressed as mean±SD.

hOX40 ICP model validation 2

hOX40 is functional on effector T cells ex vivo

Splenic and LN-isolated T cells (CD8⁺) activated with αCD3/αCD28 and immobilized hOX40L or IgG-Fc. A) Representative histogram, and B) cell proliferation determined by CFSE dilution at day 4. C) mIFN-γ production measured by ELISA in culture supernatant at day 3. Results are expressed as mean±SD. Unpaired T test analyses (Mann-Whitney ** p<0.01, *** p<0.001).

Anti-tumor efficacy and tumor-specific immunological memory were proven in MC38 and MB49 tumor models.

* For more validation data please contact us.

Preclinical Humanized OX40 Mouse Model

Design of the hOX40 mouse

Applications in immuno-oncology

hOX40 features

hOX40 validation*

Further reading

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