Humanized Mouse Models
Get supplemental information, a quote, and estimated timeframe for generating your humanized Knockin mouse.
A humanized mouse defines a Knockin model in which a mouse gene is replaced by either a human gene, genomic sequence or regulatory element.
This substitution can target parts of the gene, and subsequently specific domains of the protein, as well as certain mouse regulatory elements such as the promoter.
The human protein/domain is then expressed in cells and tissues where the mouse protein was expressed.
Typical applications for humanized Knockin mouse models
For academic research:
For bio-pharmaceutical research & development:
Strengths and limitations of humanized Knockin mouse models
- Physiological expression patterns due to the murine promoter and regulatory elements
- Murine gene is inactivated; no hybrid sequences (like, e.g., in transgenic animals)
- Combination with access to Knockout model for specificity studies
- Risk of differential expression in mouse vs. human, due to differences in protein regulation
- Low conservation between human/mouse protein => risk of hypomorphism up to protein Knockout
→ Limitation can be bypassed by using chimeric molecules to improve functionality in mouse cells
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