Humanized TNF-Alpha Mouse Model for In Vivo Preclinical Studies

Applications: inflammation and autoimmune disease

The humanized TNF-α mouse model allows in vivo efficacy and safety assessment of anti-human TNF-α compounds in the field of autoimmune diseases and inflammation.

Model features:

  • Humanization by gene substitution; the murine TNF-α gene is inactivated and replaced by the human TNF-α genomic portion
  • Human TNF-α expression is driven by the mouse TNF-α endogenous promoter (physiological TNF-α regulation and expression pattern)
  • Physiological expression level of human TNF-α (Fig. 1B)
  • No expression of murine TNF-α (Fig. 1A)
  • No physiological deregulation reported

Model validation

Fig. 1) Mean plasmatic concentrations of murine TNF-α and human TNF-α following LPS injection

TNF-alpha concentration after LPS injection

Mean concentrations of murine TNF-α (A) and human TNF-α (B) measured by ELISA from plasma samples collected from WT (n=2) and KI (n=2) mice treated with saline solution vs. LPS.

 

Ready to be shipped to your lab

  • Available onto C57BL/6J genetic background
  • Health-certified prior to delivery: SOPF/VAF Elite (Specific and Opportunistic Pathogen-Free/Virus- and Antibody-Free)
  • Worldwide delivery using logistic networks of renowned professional breeders

Access conditions

  • Freedom to operate for basic and pharmaceutical research
  • Generated data are the exclusive property of the customer and royalty-free
  • The model is available through in-licensing (flat annual fee, depending on company size)
  • On-demand production of cohorts by contract breeding