Knockout Mouse Models for Water and Ion Channels
Our catalog of KO mice contains numerous models targeting water and ion channels, voltage, ligand-gated, and others.
All our lines are submitted to a panel of phenotyping tests mandated by the International Mouse Phenotyping Consortium (IMPC) and systematically performed to identify significant phenotypes between WT and KO.
Examples of phenotyped KO lines for ion channels:
Aqp1, Aqp3, Aqp6, Grin1, Grin2d, Kcnb1, Kcne1, P2rx6,, Kcnj11, P2rx4, P2rx7…
Mutations in the human KCNB1 gene, voltage-gated potassium channel, are associated with early infantile forms of epileptic encephalopathy (1). KO mice for its homolog Kcnb1 show significant differences with WT in 4 tests performed under the IMPC guidelines, including:
KO mice for Aqp1, a water channel involved in cerebrospinal fluid secretion (2) and pain sensation (3), show significant differences with WT in 7 tests performed under the IMPC guidelines, including:
- Torkamani, A. et al. De novo KCNB1 mutations in epileptic encephalopathy. Ann. Neurol. 76, 529–540 (2014).
- Oshio, K. Reduced cerebrospinal fluid production and intracranial pressure in mice lacking choroid plexus water channel Aquaporin-1. FASEB J. (2004). doi:10.1096/fj.04-1711fje.
- Oshio, K., Watanabe, H., Yan, D., Verkman, A. S. & Manley, G. T. Impaired pain sensation in mice lacking Aquaporin-1 water channels. Biochem. Biophys. Res. Commun. 341, 1022–1028 (2006).