Chemokine & Cytokine KO Mouse Models

 

 

Our catalog of KO mice contains numerous models targeting chemokines and cytokines (see GPCR-family for chemokine receptors).

All our lines are submitted to a panel of phenotyping tests mandated by the International Mouse Phenotyping Consortium (IMPC) and systematically performed to identify significant phenotypes between WT and KO.

Examples of phenotyped KO lines for chemokines and cytokines:

Ccl22, Cxcl17, Cxcl9, Fgf10, Fgf11, Fgf2, Fgf3, Fgf7, Gfi1b, Il12a, Il15, Vegfb…

 

Example 1:

KO mice for Cxcl17, an orphan chemokine whose human homolog has tumorigenic and angiogenic activities (1), show significant differences with WT in 4 tests performed under the IMPC guidelines, including:

Figure 1b - KO mice for Cxcl17a) Abnormal heart morphology (not shown)

b) Abnormal coat/hair pigmentation in males (head, back and abdomen as assessed by Combined SHIRPA and Dysmorphology CSD)

Example 2:

KO females for Il15, a regulator of T- and NK-cell activation and proliferation in humans (2), show significant differences with WT in one behavior/neurological test performed under the IMPC guidelines:

a) Limb grasping (assessed by CSD)

Figure 1a - KO females for Il15

b) Expression data are also available for some lines, including Il15-/+ mice, showing LacZ expression in the cerebellum

Figure 1b - KO females for Il15

References:

  1. Nagarsheth, N., Wicha, M. S. & Zou, W. Chemokines in the cancer microenvironment and their relevance in cancer immunotherapy. Nat. Rev. Immunol. (2017). doi:10.1038/nri.2017.49.
  2. Mishra, A., Sullivan, L. & Caligiuri, M. A. Molecular pathways: Interleukin-15 signaling in health and in cancer. Clin. Cancer Res. 20, 2044–2050 (2014).