Metabolic Disease Mouse Models
Our catalog of KO mice contains numerous models displaying metabolic disease characteristics that you can appreciate, thanks to a panel of phenotyping tests mandated by the International Mouse Phenotyping Consortium (IMPC) and systematically performed on our lines.
- Weight curve: to monitor body weight
- Combined SHIRPA and Dysmorphology (CSD): to detect physical, behavioral and morphological abnormalities
- Calorimetry: to provide detailed information on energy metabolism
- Intraperitoneal glucose tolerance test (IPGTT): to measure clearance of injected glucose
- Dual energy X-ray Absorptiometry (DEXA): to measure bone mineral content and body composition
Examples of phenotyped KO lines with metabolic phenotypes:
Ablim1, Acbd5, Acer1, Cbx6, Cpe, Dlg4, Ehmt1, Enc1, Fahd2a, Fbf1, Fbxo31, Gnao1, Lepr, Mcu, Mrap2, Pbx3, Pls3, Pop4, Prdm10, Smco2, Snap47, Tfeb, Trim29…
Leptin receptor (LEPR) deficiency is a cause of severe obesity in humans (1). KO mice for the murine homolog Lepr, a common model for obesity and diabetes (2), show significant differences with WT in more than 30 tests performed under the IMPC guidelines, including:
About 45 mouse lines KO for genes such as Ap3s1, Cpe, Golga3, Ivd, Kntc1, Nol8, Rilpl1, Tnfaip1, Usp47, Zgpat...
About 41 mouse lines KO for genes such as Cpt1c, Eomes, Htr2b, Kptn, Myo15, Pde8a, Prpf31, Syt1, Txnip, Xxylt1...
Increased total body fat amount in unconventional myosin Myo15-/- mice (ratio fat/body weight measured by DEXA)
- Thompson, D. B., Ravussin, E., Bennett, P. H. & Bogardus, C. Structure and sequence variation at the human leptin receptor gene in lean and obese Pima Indians. Hum. Mol. Genet. 6, 675–679 (1997).
- Chen, H. et al. Evidence that the diabetes gene encodes the leptin receptor: Identification of a mutation in the leptin receptor gene in db/db mice. Cell 84, 491–495 (1996).