Metabolic Disease Mouse Models

 

 

Our catalog of KO mice contains numerous models displaying metabolic disease characteristics that you can appreciate, thanks to a panel of phenotyping tests mandated by the International Mouse Phenotyping Consortium (IMPC) and systematically performed on our lines.

Metabolism-related tests:

Examples of phenotyped KO lines with metabolic phenotypes:

Ablim1, Acbd5, Acer1, Cbx6, Cpe, Dlg4, Ehmt1, Enc1, Fahd2a, Fbf1, Fbxo31, Gnao1, Lepr, Mcu, Mrap2, Pbx3, Pls3, Pop4, Prdm10, Smco2, Snap47, Tfeb, Trim29…

 

KO of genes associated with metabolic disorders
such as obesity or diabetes

Example 1:

Leptin receptor (LEPR) deficiency is a cause of severe obesity in humans (1). KO mice for the murine homolog Lepr, a common model for obesity and diabetes (2), show significant differences with WT in more than 30 tests performed under the IMPC guidelines, including:

a) Increased circulating glucose level (measured by clinical chemistry and IPGTT)

Figure 1a - KO with metabolic disorders

b) Increased total body fat amount (quantified by DEXA)

Figure 1b - KO with metabolic disorders

c) Increased circulating cholesterol level (measured by clinical chemistry and IPGTT)

Figure 1c - KO with metabolic disorders

 

KO exhibiting increased circulating glucose level,
a phenotypic similarity to diabetes

About 45 mouse lines KO for genes such as Ap3s1, Cpe, Golga3, Ivd, Kntc1, Nol8, Rilpl1, Tnfaip1, Usp47, Zgpat...

Example 2:

a) Increased fasted circulating glucose level in adaptor protein Ap3s1-/- mice (assessed by IPGTT)

Figure 2a - KO with increased circulating glucose level

b) Expression data are also available for some lines, including Ap3s1-/+ mice, showing strong LacZ expression in the brain.

Figure 2b - KO with increased circulating glucose level

KO exhibiting increased total body fat amount,
a phenotypic similarity to morbid obesity

Figure - KO with increased total body fat amount

 

About 41 mouse lines KO for genes such as Cpt1c, Eomes, Htr2b, Kptn, Myo15, Pde8a, Prpf31, Syt1, Txnip, Xxylt1...

 

Example 3:

Increased total body fat amount in unconventional myosin Myo15-/- mice (ratio fat/body weight measured by DEXA)

References:

  1. Thompson, D. B., Ravussin, E., Bennett, P. H. & Bogardus, C. Structure and sequence variation at the human leptin receptor gene in lean and obese Pima Indians. Hum. Mol. Genet. 6, 675–679 (1997).
  2. Chen, H. et al. Evidence that the diabetes gene encodes the leptin receptor: Identification of a mutation in the leptin receptor gene in db/db mice. Cell 84, 491–495 (1996).