Oncology Mouse Models

 

 

Our catalog of KO mice contains numerous models linked to oncological studies that you can appreciate, thanks to a panel of phenotyping tests mandated by the International Mouse Phenotyping Consortium (IMPC) and systematically performed on our lines.

Examples of ready-to-use KO lines for genes linked to cancer:

Aktip, Arpc3, Bap1, Cdc26, Cdkn2a (Arf), Chek2, Ikbkb, Notch1, Pak1, Pdgfrb, Rasa1, Wee1…

 

KO of genes linked to cancer

Example 1:

In humans, BAP1 (Brca1-associated protein-1) was identified as a cancer predisposition gene (1). KO and Het mice for its murine homolog Bap1 show significant differences with WT in 5 tests performed under the IMPC guidelines, including:

Preweaning lethality with complete penetrance for Bap1-/- mice (no KO recovered during the viability primary screen)

 

Figure - KO linked to cancer

Image: Increased monocyte cell number for Bap1-/+ mice, as % of leukocytes (assessed by whole blood peripheral blood leukocyte immunophenotyping)

 

KO lines for cancer therapeutic targets

Figure - KO for cancer therapeutic targetsPhenotyped KO lines for multiple cancer therapeutic targets such as Aktip, Ikbkb, Notch1, Pdgfrb, or Wee1

 

Example 2:

Homozygous KO mice for Wee1, nuclear kinase and a therapeutic target for mutant-RAS-driven acute leukemia exhibit embryonic and preweaning lethality, whereas Wee1-/+ males show decreased circulating serum albumin levels (clinical blood chemistry).

KO lines for cell cycle progression genes

Phenotyped KO lines for genes involved in cell cycle progression, including Arpc3, Cdc26, Cdkn2a (Arf), Chek2, Pak1 or Rasa1

Example 3:

Homozygous KO mice for Rasa1, a GTPase-activating protein acting on Ras p21, whose mutation is associated with basal cell carcinoma, exhibit preweaning lethality with complete penetrance, whereas Rasa1-/+ mice present:

a) Increased body length (assessed by body composition DEXA)

Figure 1a - KO for cell cycle progression genes

b) Increased bone area (DEXA)

Figure 1b - KO for cell cycle progression genes

References:

  1. Testa, J. R. et al. Germline BAP1 mutations predispose to malignant mesothelioma. Nat. Genet. 43, 1022–1025 (2011).