Cell Line Engineering

With genOway, you directly benefit from our know-how cell line engineering, derived from generating literally thousands of genetically modified cell models for gene Knockouts, humanizations, point mutations, overexpression, and much more.

In addition, we provide you this personalized service:

  • Thorough project assessment and scientific analysis - Because each gene differs in structure and regulation and each protein in maturation and action, it is essential to fully adapt the design of the genetically modified model to the gene and protein characteristics.

  • Project specificities - To create the model most relevant to your research project, we work with you to adapt a strategy to meet your time lines, budget, intermediate results requirements, read-outs, collaborations - whatever your needs are.

  • Modifications of immortalized cell lines and stem cells - A major benefit for you and your research is our rigorous procedure to quickly evaluate the feasibility of using your proper cell line. Our genetic modifications to your chosen in-house cell line optimize the use of your existing data and avoid the need for new standardization experiments.

  • Off-target measurement - When using nucleases, it is important to assess off-target events, as shown by literature data ranging from massive to zero off-target effects. genOway has optimized protocols to assess and reduce such off target effects for your benefit. (More information here.)

  • Short development time - Miniaturization, automation and refinement of every production step using Lean management, enable us to continually improve our highly optimized and standardized production platform. The direct benefit to you? This guarantees fluid work-flow and reduces development timelines.

  • Phenotyping service - Our phenotypic characterization (quantitative measurements, physiological response read-outs, etc.) of your genetically modified model facilitates your research, providing you with faster data and results.

  • Freedom to operate (FTO) - genOway possesses a complete portfolio of intellectual property (IP) rights including exclusive IP rights on key technologies. We are therefore able to provide you with the IP rights you need in order to use your model for basic research as well as pharmaceutical applications.

Obtain the model you need:
  • genOway has developed a complete technology portfolio, enabling us to offer the most appropriate technology for each project, from homologous recombination to cassette exchange, from conditional approach to inducible systems, and much more. Our commitment is to provide you with the precise model you need. We will never settle for simply providing a model we can easily make or access. Your success is our success.


Cell Line Engineering Services

Our cell service offer is available for mammalian immortalized lines and primary or pluripotent stem cell lines.

We offer Knockouts; gene modifications such as tagging, nucleotide substitution, sequence swapping, integration, or replacement; and a comprehensive phenotyping of genetic modifications on RNA and protein levels.

Types of gene modifications for cell line engineering

Gene Knockout Service

We work with you to design the most appropriate strategy for your project characteristics in order to generate the most adapted Knockout cell lines. How? By:

  • Introducing mutations to disrupt the coding frame
  • Inserting an exogenous cassette to prevent gene expression
  • Deleting coding gene sequences

Our phentoyping services include:

  • Measuring RNA (by RT-PCR and/or RT-qPCR) and protein expression (by microscopy, cytometry and/or biochemistry) to confirm the knockout
  • Functional tests to assess the loss of protein activity (e.g., cell health assays, organelle and macrostructure labeling, migration, invasion, adhesion, etc.)

Gene Modification Service

Scientific applications for gene modifications in cells are virtually unlimited. Contingent up on your scientific strategy and project characteristics, we design and generate the most appropriate cell line, such as:

  • Gene tagging to enable simple, reliable tracking and monitoring
  • Nucleotide substitution to decipher molecular mechanisms
  • Promoter or regulatory sequence swapping or deletion to provide a powerful tool to decipher in vivo regulatory mechanisms
  • Mutation or DNA fragment introduction
  • DNA fragment replacement
  • DNA fragment deletion

Phentoyping services include:

  • Measuring RNA (by RT-PCR and/or RT-qPCR) and protein expression (by microscopy, cytometry and/or biochemistry) to confirm proper expression of the desired sequence
  • Functional tests to assess the protein activity (e.g., cell health assays, organelle & macrostructure labeling, migration, invasion, adhesion, etc.)
mRNA and protein quantification of human isoforms in murine cells by PCR and FACS

Quick Knockin™ into Permissive Loci HPRT, Rosa26 or AAVS1

The overexpression of RNA or proteins is unquestionably a major research tool. With this in mind, and to compensate for the increased complexity of Knockin approaches, genOway developed its Quick Knockin™ technology for both rodent and cell models. This strategy targets permissive loci, such as Rosa26, HPRT and AAVS1, reducing development time and cost thanks to ready-to-use targeting vectors. What does that mean for you? With Quick Knockin™, we can now deliver to you:

  • Stable expression pattern
  • Known copy number
  • No position effect variegation
  • No transgene silencing
  • No deregulation of neighboring genes

Phentoyping services are available to monitor expression level and functionality of the expressed mRNA or protein.

Phenotyping Service

We provide you with a comprehensive panel of cellular phenotyping tests to functionally characterize the engineered clones and compare them to the unmodified cell line by means of:
  • Determination of the expression of your target gene on mRNA level by RT-PCR and/or RT-qPCR
  • Target protein profiling by microscopy, cytometry and biochemistry
  • Functional assessment of the genetic modification at protein level by reliable standard in vitro assays (cell health assays, organelle and macrostructure labeling, migration, invasion and adhesion assays, metabolism and morphometry, secretion assays, and protein translocation)


Project Assessment & Development Process