Time-dependent conditional Knockout mouse

A time-dependent conditional Knockout mouse defines an inducible animal model in which a gene of interest is "floxed" thus temporally controllable at a given time-point in embryonic, post-natal or adult animals.

After an additional breeding step with a Cre-ERt2 deleter mouse line, the conditional Knockout is temporally triggered by external inducer-agents, most often small molecules such as tamoxifen or tetracycline.

Applications

For academic research:

• Comparative study of gene function (on- vs. offset)
• Mimic a pathology with late onset
• Studying embryonic lethal conditions

For bio-pharmaceutical research & development:

• Target validation studies
• Off-target effects studies
• Mimicking 100% antagonism
• Compensatory phenotype rescue (compounds testing)