FLEx: Reference technology for inducible point mutations

The FLEx technology is gold standard for inducible point mutations leading to kinase-dead and functional Knockouts, in a temporal- and tissue-specific manner.

FLEx technology allows scientists to induce the expression of a mutated gene or a reporter gene at an appropriate time during the lifetime of the animal model.

FLEx technology was invented by Prof. Chambon, Dr. Schnütgen and Dr. Ghyselinck at the Institut de Génétique et de Biologie Moléculaire et Cellulaire (France).

Access to time- and tissue-restricted point mutant models

This technique has become the gold standard for the generation of kinase-dead mutants and functional Knockouts. The mutant gene is induced in a temporal- and tissue-specific manner. Mutant gene expression is physiologically relevant and appropriate since the gene continues to be expressed from its endogenous locus.

The FLEx model:

  • Bypasses potentially lethal phenotypes associated with the expression of the mutation
  • Allows the researcher to analyze the effect of the mutated gene when the mouse is an adult
  • Can reproduce a particular pathology that is triggered by a mutation and that normally manifests in adulthood
  • Expresses the mutation from the endogenous locus and can therefore be regulated physiologically

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Access to monitoring of tissue-specific and temporally specific gene ablation

FLEx technology can also create conditional mouse models for the express purpose of monitoring gene deletion.

This approach is often valuable when:

  • No reliable antibody exists that can detect your target gene or quantify its deletion
  • You are interested in monitoring the trafficking of gene-deleted cells
  • You wish to monitor gene expression patterns

How does it work?

This tool is based on the Cre-lox system.

Both loxP and lox511 are recognized by Cre recombinase, but lox511 sites can only recombine with other lox511 sites, not with loxP sites.

When the DNA sequence is flanked by lox sites in opposing orientations, the Cre will invert the sequence between the sites. If the DNA sequence is flanked by lox sites in the same orientation, the Cre will excise the sequence.

By combining the type, number, and orientation of lox sites that surround your genes of interest, a powerful FLEx tool can be created, tailored to your specific scientific needs.

FLEx: cre-lox system

References describing applications using FLEx technology:

Inducible Knockout: F. Schnütgen et al. Nature Biotech 2003. A directional strategy for monitoring Cre-mediated recombination at the cellular level in the mouse.

Gene trapping: F. Schnütgen et al. PNAS 2005. Genomewide production of multipurpose alleles for the functional analysis of the mouse genome.

Gene reporter switch: D. Atasoy et al. J Neuroscience 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping.

genOway licenses secure your discoveries and research

genOway holds an exclusive license from the University of Strasbourg, Inserm and CNRS, France. Patents for this technology are issued and maintained in the US (US7074611) and in Europe (EP1383891).

You, the customer, has a permanent, non-exclusive and royalty-free license for the patented and/or proprietary technologies employed to create the rodent model. This guarantees you full rights to use such a model for any and all R&D purposes.

You also retain ownership of the deliverables and can patent the model developed. genOway has no claim on the results generated using the model we provide for you.

Other technologies

RMCE: Recombinase-Mediated Cassette Exchange

Recombinase-mediated cassette exchange enables the swapping of large genomic regions and is recommended for the generation of humanized models.

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SSR: Site-Specific Recombination

Site-specific recombination is a gene engineering tool (e.g., Cre-lox and FLP-FRT systems) that relies on recombinases to replace specific DNA sequences.

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TET System: Controlled gene expression

Use tetracycline for reversible and efficient spatiotemporal control of gene expression. This on-demand gene induction mimics disease onset and progression.

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Homologous Recombination

Homologous recombination: A robust and efficient gene targeting technology for humanized, Knockin & conditional Knockout models for human disease studies.

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SMASh: A drug-inducible protein turnover control system

This drug-inducible and reversible protein degradation system is of particular interest to model-targeted protein degradation therapeutic approaches in the preclinical stage.

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IRES: Internal ribosome entry site

IRES allows to co-express of several genes under the control of the same promoter and is considered to be the "go-to" technology for transgene co-expressions.

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CRISPR/Cas9 gene editing

CRISPR/Cas9: structure, mechanisms of action, efficacy, off-target activity, genetic backgrounds, multi-targeting, point-mutations, and large deletions.

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