Point mutation cell lines

A point mutation Knockin cell line defines a model in which one or more nucleotides are constitutively mutated.

The insertion, deletion, nonsense and sense mutations alter the amino acid sequence of a given protein, thus changing its functions or properties.

Applications

For academic research:

• Decipher protein function (gain or loss of function)‍
• In vitro recapitulation of a human disease

For bio-pharmaceutical research & development:

• Pharmacological off-target and efficacy studies by suppressing xenobiotics or antibody binding
• Study drug-resistant mutants
• Synthetic mortality screening in cancers