A bicomponent CRISPR‑Cas9 system induces sex-specific embryonic lethality via targeting Top1, enabling generation of all-male or all-female litters with 100% efficiency and litter size compensation. The system can also generate postnatal sex-specific phenotypes.
Co‑inheritance leads to elimination of male or female embryos depending on Cas9‑chromosome; 100% single‑sex litters; litter sizes exceed 50% of controls; system repurposable for sex‑specific phenotypes
Top1 sgRNA Knockin (autosomal H11 locus) combined with Cas9 Knockin (X‑ or Y‑linked) — genOway-developed bicomponent CRISPR‑Cas9 Knockin mouse system
Genetic sex selection; 3Rs reduction of animal use; synthetic lethality; sex‑specific gene editing; developmental biology
Synthetic lethal bicomponent CRISPR‑Cas9 system; IRES‑mCherry reporter; autosomal H11‑Top1 sgRNA Knockin; X‑Cas9 or Y‑Cas9 Knockin; embryonic lethality; fluorescent tracking; breeding strategy
From model design to experimental results
Featured in 600+ scientific articles
Collaboration with 17 Top Pharmas,
170+ Biotechs and 380+ Academic Institutions
Generated with biopharma partners and in-house
and guaranteed freedom to operate
Models with certified health status from professional breeders in US and Europe
