This study developed and validated a Knockin mouse model expressing Cre recombinase under the control of the endogenous Nmu promoter. Utilizing this model, researchers performed a comprehensive mapping of NMU expression in the adult mouse brain, unveiling a potential midline NMU modulatory circuit with the VMH as a key node.
The study provided a detailed neuroanatomical map of NMU-expressing neurons, identified a midline NMU modulatory circuit with the VMH as a central node, and demonstrated that NMU neurons in the VMH primarily constitute a unique population of hypothalamic cells.
Nmu-IRES-Cre Knockin mouse model developed by genOway, where an IRES (internal ribosome entry site) sequence was inserted downstream of the Nmu coding sequence, allowing bicistronic expression of NMU and Cre recombinase from a single mRNA transcript. This model enables precise genetic targeting of NMU-expressing neurons without disrupting endogenous NMU expression.
Neuropeptide mapping, Hypothalamic circuits, Neuronal characterization, NMU function
Knockin mouse model, Cre-loxP system, IRES-mediated bicistronic expression, Neuropeptidergic neuron mapping, Nmu promoter targeting
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
