This study investigates the role of Panton-Valentine leukocidin (PVL), a toxin produced by Staphylococcus aureus, in the severity of implant-associated osteomyelitis. Using humanized BRGSF mice, the research examines how PVL influences infection outcomes, immune cell dynamics, and bacterial dissemination.
Mice infected with PVL-producing S aureus strains exhibited more severe clinical outcomes, including higher bacterial loads in bone and soft tissues, formation of staphylococcal abscess communities in bone marrow, and increased dissemination to internal organs. These findings highlight the pivotal role of PVL in exacerbating implant-associated osteomyelitis.
The study utilized humanized BRGSF mice, which are immunodeficient mice engrafted with human hematopoietic stem cells, leading to the development of a human-like immune system. This model allows for the assessment of human-specific immune responses to S. aureus infection.
Osteomyelitis, Staphylococcus aureus, Panton-Valentine leukocidin, Humanized mouse model, Implant-associated infections
Humanized BRGSF mice, Hematopoietic stem cell engraftment, Immune response assessment, Bacterial pathogenesis, Infection modeling
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
