Validation
MC38-hPD-L1-LZ cells express homogeneous high levels of human PD-L1 and ZsGreen
Expression analysis by flow cytometry. hPD-L1 is expressed in MC38-hPD-L1 (left panel) and MC38-hPD-L1-LZ cells (right panel). MC38-hPD-L1-LZ cells also express ZsGreen.
MC38-hPD-L1-LZ cells form tumors in vivo, and are detectable by luminescence imaging
Wild-type C57BL/6 mice were injected (s.c.) with 1x105 MC38 or MC38-hPD-L1-LZ cells. (A) Tumor growth was measured every 3 days. Tumor size (mm3) = (width2 x length)/2. 6 mice were injected per group, with 5 mice developing tumors for MC38 and MC38-hPD-L1-LZ injected groups. (B) Mice received injections (i.p) of D-Luciferin (150mg/kg) and were imaged 15 minutes later under isoflurane anaesthesia on an IVIS Lumina II imager. Note that tumors can be detected by luminescence imaging before they are palpable (D8).
Humanized MC38-hPD-L1-LZ tumor cell line
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