Related resources and publications
Targeting the human CD47/SIRPα axis
Humanized ICP mouse and cell lines
Front Immunol
PLoS One
Immunology
The MC38-hPD-L1-LZ clonal cell line expresses high levels of human PD-L1 and forms solid tumors in vivo. An optimized version of a luciferase-ZsGreen (LZ) fusion protein is also stably expressed for in vivo imaging and ex vivo tracking.
We generated the MC38-hPD-L1-LZ clonal cell line expressing high levels of human PD-L1 and forming solid tumors in vivo. An optimized version of a luciferase-ZsGreen (LZ) fusion protein is also stably expressed for in vivo imaging and ex vivo tracking.
Expression analysis by flow cytometry. hPD-L1 is expressed in MC38-hPD-L1 (left panel) and MC38-hPD-L1-LZ cells (right panel). MC38-hPD-L1-LZ cells also express ZsGreen.
Wild-type C57BL/6 mice were injected (s.c.) with 1x105 MC38 or MC38-hPD-L1-LZ cells. (A) Tumor growth was measured every 3 days. Tumor size (mm3) = (width2 x length)/2. 6 mice were injected per group, with 5 mice developing tumors for MC38 and MC38-hPD-L1-LZ injected groups. (B) Mice received injections (i.p) of D-Luciferin (150mg/kg) and were imaged 15 minutes later under isoflurane anaesthesia on an IVIS Lumina II imager. Note that tumors can be detected by luminescence imaging before they are palpable (D8).
Targeting the human CD47/SIRPα axis
Humanized ICP mouse and cell lines
Front Immunol
PLoS One
Immunology
Let us know how we can help