Immunodeficient & Reconstituted Mouse & Rat Strains
Our immunodeficient mouse model portfolio is based on the proprietary state-of-the-art BRGS mouse model.
Compared to traditional immunodeficient strains, such as NOD-SCID and NSG, the BRGSF mouse represents the most immunodeficient model generated to date, with defect of both the murine lymphoid and myeloid compartments, and thus represents a strongly adapted animal model for xenograft of human tumor and/or immune system.
The portfolio consists of the following strains:
- BRGS (BALB/c Rag2tm1Fwa Il2rgtm1Cgn SirpaNOD)
- BRGSF (BALB/c Rag2tm1Fwa Il2rgtm1Cgn SirpaNOD Flt3tm1lrl)
- BRGSF-A2 (genetically humanized for HLA-A2HHD–Tg(HLA-A/H2-D/B2M)1Bpe)
Immunodeficient humanized mouse models offer new opportunities to address human-specific questions in a more relevant manner, facilitating translational research in several biomedical disciplines and approaches.
Our BRGSF mice are excellent recipients for human hematopoietic cells. We routinely generate BRGSF-HIS (humanized for the immune system) mice, using human CD34+ hematopoietic progenitor cells.
The BRGSF-HIS mice contain all major human hematopoietic cell subsets, such as B cells, T cells (including CD4+ Treg cells), NK cells, and the myeloid compartment including dendritic cells (DCs), plasmacytoid cells (pDCs) and monocytes/macrophages.
The BRGSF-A2-HIS model is generated by inserting an human leukocyte antigen serotype A2 (HLA-A2) class I transgene in the genome of BRGSF (BALB/c Rag2tm1Fwa Il2rgtm1Cgn SirpaNOD Flt3tm1lrl) mice, and subsequently reconstituted with a functional human immune system (HIS).
Compared to the traditional immunodeficient nude rats, which lack T cells but maintain a normal repertoire of all other immune cells, including B and NK cells, the SDRG model is a double Knockout for Rag1 and IL-2Rγ genes (Rag1-/- Il2rγ-/-), resulting in a B, T and NK cell deficiency.