A tissue-specific Knockout rat defines an animal model in which a gene of interest is "floxed" and thus inactivatable in specific cell types in a certain tissue (conditional). Other cell types and tissues exhibit an unmodified, functional gene expression.

This gene inactivation is achieved by an additional breeding step with a tissue- or cell type-specific Cre-deleter rat line. The creation process of such models also gives access to whole body (constitutive) Knockouts

Applications

For academic research:

  • Study gene function in one specific organ, tissue or cell type
  • Mimic pathologies caused by gene inactivation in a given cell type
  • Create a tissue-specific phenotype for multi-function proteins

For bio-pharmaceutical research & development:

  • Validate target genes with cell-specific functions
  • Investigate signaling pathways in certain cell types
  • Safety studies

Strengths of tissue-specific Knockout rat models

  • Very flexible: easy switch to study another tissue
  • High physiological relevancy of the obtained scientific data from such a model
  • Enables access as well to constitutive Knockout for comparison studies

Limitations of tissue-specific Knockout rat models

  • Rat line creation requires the insertion of exogenous sequences (loxP, FRT) that can deregulate transcription and splicing
    → Risk can be greatly minimized by applying in-depth bio-informatic, genetic, and bibliographic analysis
  • Few deleter rats are available, requiring the creation of new Cre-deleter rats
    → New tissue- or cell-specific Cre rat lines can be produced by genOway in parallel to the conditional rat model
  • Inactivation of the gene of interest in one cell type may occur during cell type differentiation, resulting in an impaired phenotype and/or modified cell physiology
    →  Limitation can be bypassed by applying conditions such as time-specific gene inactivation
  • To obtain tissue specificity, a crossing step with a deleter rat line implies a time-consuming production of cohorts
    →  This "time" issue must be anticipated and integrated in the scientific consulting prior to model development
Related ressources et publication
No results
No results
No items found.
No results
No results
No results
No results

Discover related products to

Tissue-specific KO rats

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
Point mutation
Customized
Knockin
Rats

Point mutation KI rats

Use a point mutation rat Knockin to circumvent complex phenotypes arising from complete Knockouts (e.g., signaling pathway problems, cross-reactivity).

Knockin
Permissive locus
Customized
Rats

Quick KI rats

The Rosa26 and Hprt gene loci are well suited for gene over-expression, reduced development time and cost with ready-to-use targeting vectors.

Knockout
Constitutive
Customized
Rats

Constitutive KO rats

Use a constitutive Knockout rat to permanently inactivate your target gene; in the whole animal, in every cell of the organism, effective at all stages of development, from the one-cell embryo stage through adulthood.

Knockout
Conditional
Time-dependent
Customized
Rats

Time-dependent KO rats

Use an inducible conditional Knockout rat to age-dependently inactivate your gene, and to enable studies at defined development stages or on age-related diseases.

Discover our off-the-shelf models.

Learn more

Tissue-Specific conditional Knockout rat models

Get in touch about

Let us know how we can help

In order to provide you the content requested, we need to store and process your personal data. If you consent to us storing your personal data for this purpose, please tick the checkbox below.

From time to time, we would like to contact you about our products and services, as well as other content that may be of interest to you. If you consent to us contacting you for this purpose, please tick below.

You can unsubscribe from these communications at any time. For more information on how to unsubscribe, our privacy practices, and how we are committed to protecting and respecting your privacy, please review our Privacy Policy.

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.