Transgenic / Genetically Modified Research Models
Traditionally, transgenic models refer to genetically modified (GM) organisms in which the genome has been altered through random integration of gene sequences.
Today, the wording is used in a broader sense for all kinds of targeted gene mutations, like:
- Deletion (Knockout)
- Insertion/amplification (Knockin)
- Substitution (e.g., point mutations and humanizations)
Models at genOway:
- Genetically modified mice (customized and off-the-shelf), rats and cell lines
- Gene Knockout, Knockin, point mutation and humanization
- Inducible and constitutive expression
Technologies we use:
- Homologous recombination (HR) and nuclease-based gene editing (e.g. CRISPR/Cas9)
- Site-specific recombination (SSR), including RMCE, FLEx, and Cre-ERt2
- Tetracycline-based systems (e.g., Tet-On/Off)
- Internal ribosome entry site (IRES)
Intellectual property:
We own licenses on all technlogies used to develop your model. This provides you the freedom to operate on your model, and allows you to retain ownership and patent it. (License your model.)
Our customized transgenic mouse, rat and cell line models
Customized mouse models
Follow the links to navigate to customized mouse models we can develop for you.*
| Knockout mouse model | |
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Constitutive KO Permanent inactivation of the target gene, and total absence of the protein in the whole animal, in every cell of the organism |
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| Conditional KO | |
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Tissue-specific KO Gene of interest is inactivated in specific cell types in a certain tissue |
Inducible KO Gene of interest can be temporally controlled at a given time-point in embryonic, post-natal or adult animals |
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Functional KO One or more mutated nucleotides causing loss of function of a given protein |
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| Knockin mouse model |
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Constitutive KI Add a gene sequence of interest to an endogenous locus |
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Quick KI Controlled gene expression by targeting permissive loci Rosa26 or Hprt |
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Point mutation KI One or more constitutively mutated nucleotides affecting the function of a given protein (gain/loss of function) |
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Reporter KI With inserted fluorescent, bioluminescent proteins or biochemical tags |
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Humanization KI Substitute a mouse gene sequence with the human counterpart |
* In addition, we are running, in collaboration with customers, several R&D programs for other than the above mouse model developments. If you are interested in joining such a program, please reach out to us.
Customized rat models
Follow the links to navigate to customized rat models we can develop for you.*
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Permanent inactivation of the target gene, and total absence of the protein in the whole animal, in every cell of the organism |
One or more constitutively mutated nucleotides affecting the function of a given protein (gain/loss of function) |
* In addition, we are running, in collaboration with customers, several R&D programs for other than the above rat model developments. If you are interested in joining such a program, please reach out to us.
Custom cell line models
Follow the links to navigate to custom cell line models we can develop for you.*
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Constitutive Knockouts Permanent inactivation of the target gene, and total absence of the protein |
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Point mutation Knockins One or more constitutively mutated nucleotides affecting the function of a given protein (gain/loss of function) |
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Reporter Knockins With inserted fluorescent, bioluminescent proteins or biochemical tags |
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Quick Knockin™ Controlled gene expression by targeting permissive loci Rosa26, Hprt or AAVS1 |
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"Simple" Knockin Add a gene sequence of interest to an endogenous locus |
* In addition, we are running, in collaboration with customers, several R&D programs for cell line developments. If you are interested in joining such a program, please reach out to us.
Our repository and off-the-shelf transgenic mouse models
Repository of more than 2000 Knockout mice
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More than 2000 ready-to-use Knockout models for:
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Categorized into therapeutic areas: Categorized into gene families: |
Off-the-shelf mice
Humanized models
- Double humanization for neonatal Fc receptor and albumin
- Measure and optimize albumin-linked small molecules and conventional drug pharmacokinetics
- Study and predict the half-life of circulating biologics and biosimilar drugs
- In vivo efficacy and safety assessment of anti-human CD4 compounds
- Typically used in the fields of inflammation, autoimmune diseases, immuno-oncology, HIV infection
- Double humanization for IgE and Fcε receptor 1
- Screen for innovative therapeutics for allergy, asthma and other IgE-mediated diseases
- In vivo efficacy and safety assessment of anti-human TNF-α compounds in the field of autoimmune diseases and inflammation
Reporter models
- Reporter model to monitor immune response polarization
- Monitor and sort IL-4-secreting cells
- Reporter model to monitor regulatory T cell activity
- Monitor and sort Foxp3-expressing cells
- Study immune tolerance and checkpoint inhibition