Customized Transgenic / Genetically Modified Research Models

Transgenic models

Customized genetically modified (transgenic) models heavily impact the scientific value of your studies, as the models are designed to specifically address your scientific questions while avoiding the unrevealed caveats possible in genetically modified models.

Models at genOway:

  • Genetically modified micerats and cell lines
  • Gene Knockout, Knockin, point mutation and humanization
  • Inducible and constitutive expression

Technologies we use:

  • Homologous recombination (HR) and nuclease-based gene editing (e.g. CRISPR/Cas9)
  • Site-specific recombination (SSR), including RMCEFLEx, and Cre-ERt2
  • Tetracycline-based systems (e.g., Tet-On/Off)
  • Internal ribosome entry site (IRES)

Intellectual property:

We own licenses on all technlogies used to develop your model. This provides you the freedom to operate on your model, and allows you to retain ownership and patent it. (License your model.)

 

Our customized transgenic mouse, rat and cell line models

Customized mouse models

Follow the links to navigate to customized mouse models we can develop for you.*

Knockout mouse model
Constitutive KO
Permanent inactivation of the target gene, and total absence of the protein in the whole animal, in every cell of the organism
Conditional KO
Tissue-specific KO
Gene of interest is inactivated in specific cell types in a certain tissue
Inducible KO
Gene of interest can be temporally controlled at a given time-point in embryonic, post-natal or adult animals
Functional KO
One or more mutated nucleotides causing loss of function of a given protein

Knockin mouse model
Constitutive KI
Add a gene sequence of interest to an endogenous locus
Quick KI
Controlled gene expression by targeting permissive loci Rosa26 or Hprt
Point mutation KI
One or more constitutively mutated nucleotides affecting the function of a given protein (gain/loss of function)
Reporter KI
With inserted fluorescent, bioluminescent proteins or biochemical tags
Humanization KI
Substitute a mouse gene sequence with the human counterpart

* In addition, we are running, in collaboration with customers, several R&D programs for other than the above mouse model developments. If you are interested in joining such a program, please reach out to us.

Customized rat models

Follow the links to navigate to customized rat models we can develop for you.*

Constitutive Knockout
Permanent inactivation of the target gene, and total absence of the protein in the whole animal, in every cell of the organism

Conditional Knockout

Tissue-specific Knockout
Gene of interest is inactivated in specific cell types in a certain tissue

Inducible Knockout
Gene of interest can be temporally controlled at a given time-point in embryonic, post-natal or adult animals

Point mutation Knockins
One or more mutated nucleotides causing gain or loss of function of a given protein

Quick Knockin™
Controlled gene expression/copy number by targeting the permissive (neutral) locus Rosa26

* We are running, in collaboration with customers, several R&D programs to develop more challenging rat models. If you are interested in developing innovative rat models through partnerships, please reach out to us.

Custom cell line models

Follow the links to navigate to custom cell line models we can develop for you.*

Constitutive Knockouts
Permanent inactivation of the target gene, and total absence of the protein
Point mutation Knockins
One or more constitutively mutated nucleotides affecting the function of a given protein (gain/loss of function)
Reporter Knockins
With inserted fluorescent, bioluminescent proteins or biochemical tags
Quick Knockin™
Controlled gene expression by targeting permissive loci Rosa26, Hprt or AAVS1
"Simple" Knockin
Add a gene sequence of interest to an endogenous locus

* In addition, we are running, in collaboration with customers, several R&D programs for cell line developments. If you are interested in joining such a program, please reach out to us.