Transgenic / Genetically Modified Research Models

Transgenic models

Traditionally, transgenic models refer to genetically modified (GM) organisms in which the genome has been altered through random integration of gene sequences.

Today, the wording is used in a broader sense for all kinds of targeted gene mutations, like:

  • Deletion (Knockout)
  • Insertion/amplification (Knockin)
  • Substitution (e.g., point mutations and humanizations)

Models at genOway:

  • Genetically modified mice (customized and off-the-shelf), rats and cell lines
  • Gene Knockout, Knockin, point mutation and humanization
  • Inducible and constitutive expression

Technologies we use:

  • Homologous recombination (HR) and nuclease-based gene editing (e.g. CRISPR/Cas9)
  • Site-specific recombination (SSR), including RMCE, FLEx, and Cre-ERt2
  • Tetracycline-based systems (e.g., Tet-On/Off)
  • Internal ribosome entry site (IRES)

Intellectual property:

We own licenses on all technlogies used to develop your model. This provides you the freedom to operate on your model, and allows you to retain ownership and patent it. (License your model.)

 

Our customized transgenic mouse, rat and cell line models

Customized mouse models

Follow the links to navigate to customized mouse models we can develop for you.*

Knockout mouse model
Constitutive KO
Permanent inactivation of the target gene, and total absence of the protein in the whole animal, in every cell of the organism
Conditional KO
Tissue-specific KO
Gene of interest is inactivated in specific cell types in a certain tissue		 Inducible KO
Gene of interest can be temporally controlled at a given time-point in embryonic, post-natal or adult animals
Functional KO
One or more mutated nucleotides causing loss of function of a given protein

Knockin mouse model
Constitutive KI
Add a gene sequence of interest to an endogenous locus
Quick KI
Controlled gene expression by targeting permissive loci Rosa26 or Hprt
Point mutation KI
One or more constitutively mutated nucleotides affecting the function of a given protein (gain/loss of function)
Reporter KI
With inserted fluorescent, bioluminescent proteins or biochemical tags
Humanization KI
Substitute a mouse gene sequence with the human counterpart

* In addition, we are running, in collaboration with customers, several R&D programs for other than the above mouse model developments. If you are interested in joining such a program, please reach out to us.

Customized rat models

Follow the links to navigate to customized rat models we can develop for you.*

Constitutive Knockout
Permanent inactivation of the target gene, and total absence of the protein in the whole animal, in every cell of the organism

Conditional Knockout

Tissue-specific Knockout
Gene of interest is inactivated in specific cell types in a certain tissue

Inducible Knockout
Gene of interest can be temporally controlled at a given time-point in embryonic, post-natal or adult animals

Point mutation Knockins
One or more mutated nucleotides causing gain or loss of function of a given protein

Quick Knockin™
Controlled gene expression/copy number by targeting the permissive (neutral) locus Rosa26

* We are running, in collaboration with customers, several R&D programs to develop more challenging rat models. If you are interested in developing innovative rat models through partnerships, please reach out to us.

Custom cell line models

Follow the links to navigate to custom cell line models we can develop for you.*

Constitutive Knockouts
Permanent inactivation of the target gene, and total absence of the protein
Point mutation Knockins
One or more constitutively mutated nucleotides affecting the function of a given protein (gain/loss of function)
Reporter Knockins
With inserted fluorescent, bioluminescent proteins or biochemical tags
Quick Knockin™
Controlled gene expression by targeting permissive loci Rosa26, Hprt or AAVS1
"Simple" Knockin
Add a gene sequence of interest to an endogenous locus

* In addition, we are running, in collaboration with customers, several R&D programs for cell line developments. If you are interested in joining such a program, please reach out to us.

 

Our repository and off-the-shelf transgenic mouse models

Repository of more than 2000 Knockout mice

More than 2000 ready-to-use Knockout models for:

  • Target discovery and target confirmation
  • In vivo compound specificity
  • Mechanism of action (MOA) studies
  • Clinical studies

Find more detailed information here.

Categorized into therapeutic areas:

Categorized into gene families:

Off-the-shelf mice

Humanized models

Immune checkpoint (ICP)

  • Humanized immunocompetent mice harboring single or double ICP target(s)
  • Suitable for studying:
    • Well-calibrated syngeneic tumor models
    • Long-term treatment(s)
    • T cell exhaustion and reminiscence

hFcRn + hAlbumin

  • Double humanization for neonatal Fc receptor and albumin
  • Measure and optimize albumin-linked small molecules and conventional drug pharmacokinetics
  • Study and predict the half-life of circulating biologics and biosimilar drugs

hCD4

  • In vivo efficacy and safety assessment of anti-human CD4 compounds
  • Typically used in the fields of inflammation, autoimmune diseases, immuno-oncology, HIV infection

hIgE + FcεR1

  • Double humanization for IgE and Fcε receptor 1
  • Screen for innovative therapeutics for allergy, asthma and other IgE-mediated diseases

hTNF-α

  • In vivo efficacy and safety assessment of anti-human TNF-α compounds in the field of autoimmune diseases and inflammation

 

Reporter models

IL-4-IRES-eGFP (4get)

  • Reporter model to monitor immune response polarization
  • Monitor and sort IL-4-secreting cells

Foxp3-IRES-mRFP (FIR)

  • Reporter model to monitor regulatory T cell activity
  • Monitor and sort Foxp3-expressing cells
  • Study immune tolerance and checkpoint inhibition