Genetically Modified Models by CRISPR/Cas9 Nuclease System

With four years of R&D and more than 100 models developed, we have designed a unique CRISPR/Cas9-based offer combining our expertise in model design and our rigorous quality controls for mouse and rat models with the advantages of gene editing technologies (cost effectiveness and short development time).


You will benefit from:
  • 3 independent genetically modified lines
  • Genetic backgrounds such as C57BL6, Spraque-Dawley, etc.
  • Freedom to operate thanks to our unique IP portfolio, including a license from Broad Institute (MIT)

 
  • Off-target measurement
  • Short development time

 

1st Milestone: Model Design and Production of CRISPR/Cas9 Nuclease.

Design of a strategy taking into account the target gene features and the customer project’s objectives.
By using our proprietary technologies, we are able to design CRISPR/Cas9 nucleases with high activity and low off-target effects (see §3 Nuclease Efficacy).

2nd Milestone: Production of Founder Mice or Rats.

Injection of CRISPR/Cas9 nucleases into fertilized embryos.
Re-implantation in foster mothers under SOPF/VAF+ conditions.
Breeding and genotyping of pups for the presence of the desired mutation with the objective to characterize three independent founders.

3rd Milestone: Production of Heterozygous Animals.

Breeding of the three independent founders.
Genotyping and characterization of three heterozygous independent lines.
Assessment of the off-target effects with the option to breed the heterozygous animals to segregate the detected off-target mutations.