BRGSF-HIS features
The BRGSF-HIS mouse model possesses all of the major human hematopoietic cell subsets, such as B cells, T cells (including CD4+Treg cells), NK cells, and the myeloid compartment, including dendritic cells (DCs), plasmacytoid cells (pDCs), and monocytes/macrophages.
- Highly permissive to human xenograft, including primary or established tumor cells (CDX or PDXs) by virtue of the SIRPαNOD allele expression
- Lifespan after humanization similar to that of the wild-type mouse ⇨ supports long-term engraftment studies
- Robustness to traveling (stable immune system)
- Suitable to assess the effects of radiation treatment in vivo due to the absence of the SCID mutation on a BALB/c background
- A complete, functional complement system makes this a powerful tool for complement-dependent cytotoxicity (CDC) studies
Validation
Effects of Flt3L-induced boost of myeloid compartment in BRGSF -HIS non-small-cell lung cancer (A549) tumor model.
25-week-old BRGSF-HIS mice were injected with hFI3/Fc orvehicle priorto A549 inoculation (DO). Tumor grovth vas followed over40 days (A), and tumors (400-500 mm) were collected and analyzed by flowcytometry. Percentage of human CD45+ cells was determined (B), and immune in filtrate composition was identified (C). T cells = hCD3+; NK cells= hCD37-/hCD19-/hCD56+; Myeloid cells = hCD3-/hCD19-/hCD56-. B cells (hCD19+) were absent from the tumors. Data from 26 mice, 3 donors.
TNBC BRGSF-HIS model is donor-independent and recapitulates TNBC patients' TME.
2- week-old BRGSF-HIS mice were injected with hFIt3/Fc prior to MDA-MB-231 inoculation (DO). Tumor growth was followed over 30 days (A), and tumors (400-500m3) were collected and analyzed by flowcytometry. Percentage of human CD45+ cells in live cells (B), human monocytes in myeloid cells (C), hCD206+ M2 macrophages in monocytes (D), and hCD80+ and/or hCD86+ activated M2 macrophages (E) were determined. T cells = hCD3+/hCD56-; NK cells = hCD3-/hCD56+; Myeloid cells = hCD3-/hCD56-/hCD11b+/hCD33+; B cells = hCD19+/hCD3-; Monocytes =hCD19-/hCD3-/hCD14+. Data from 25 mice, 5 donors.
BRGSF-HIS mouse model - humanized for immune system
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