Ensure the translatability of efficacy studies for CCR8 therapeutics

Reliably assess the efficacy of your human-specific CCR8-targeting antibodies for Treg depletion with our humanized CCR8/CCL1 models

A functional CCR8-CCL1 pathway is essential for translatable results, as it’s been shown that mouse CCR8 does not cross-react with human CCL1:

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Figure 1 : Activation of the CCR8/CCL1 pathway leads to phosphorylation of ERK2. Only human CCL1 activates human CCR8, leading to an increase in ERK2 phosphorylation.

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CCR8/CCL1 interaction has been reported to trigger:

• CCR8 upregulation in Tregs and enhancement of Treg activity
• Tregs addressing to sites of antigen presentation in secondary lymphoid tissues and inflamed areas
• STAT3-dependant up-regulation of FOXP3, CD39, IL-10 and Granzyme B

Thus, only humanizing CCR8 could result in impaired CCR8 and granzyme B upregulation and altered Treg migration to secondary lymphoid tissues and to the tumor microenvironment (TME). 

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This is why we created the genO-hCCR8/hCCL1, a model in which it has been demonstrated that you can test your human-specific CCR8 antibodies. Find out more about this data in the poster we presented at the SITC 2025 conference

If you’d like more information regarding the genO-hCCR8/hCCL1 model, you can find it below:

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Testing an antibody targeting human CCR8 with a Fc-competent domain?

You might not be capturing the contribution of the effector function of your antibody if you are not using a model with humanized Fcγ receptors.

We crossed our genO-hCCR8/hCCL1 model with the extensively validated genO-hFcγR model, to enable you to test antibodies with an Fc competent domain and acquire a more complete and translatable readout of the efficacy of your therapeutic.  

This is a unique model, featuring:  

• A human-like expression pattern of hFcγ receptors and of hCCR8 and hCCL1

• Functional receptors, enabling assessment of Fc-mediated effector functions

• Functional CCR8/CCL1 signalling axis

• No expression of murine FcγR

• A fully functional mouse immune system

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We also have other models available for testing Treg-depleting therapeutics:

• genO-BRGSF-HIS
• genO-hCTLA-4
• genO-hOX40
• genO-hGITR