This study addresses challenges in generating Poldip2 Knockout mice. Initial gene trap models exhibited perinatal lethality and unintended downregulation of adjacent genes. A new conditional floxed Poldip2 model was developed using homologous recombination. However, an unexpected 305 kb duplication occurred during gene targeting, affecting gene expression. After identifying and correcting this issue, viable homozygous Knockout mice were produced, allowing for accurate functional studies of POLDIP2.
Identification and correction of a 305 kb duplication in initial Poldip2 floxed mice; successful generation of viable homozygous Knockout mice for functional studies.
Conditional floxed Poldip2 mouse model developed by genOway using homologous recombination in ES cells; initial models had a 305 kb duplication; corrected models enabled viable homozygous Knockouts.
Gene targeting, Knockout mouse model, Vascular biology, Mitochondrial function, DNA repair
Homologous recombination, loxP-flanked exon 4, FRT-flanked neomycin cassette, Cre-loxP system, Copy number variation analysis
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
