Using CRISPR/Cas9, the authors inserted an IRES‑Cre cassette downstream of the STOP codon in exon 17 of Prkcd in rats. Cre expression is selective to PKCδ⁺ cells in the central amygdala. Anatomical tracing, electrophysiology, and behavioral assays including food intake and real‑time place preference validate functionality of the model.
Cre is expressed in PKCδ⁺ neurons; projection patterns match known circuits; optogenetic stimulation of PKCδ⁺ neurons in CeA modulates feeding latency and aversion without altering locomotion or baseline behaviors
Prkcd IRES-Cre Knockin rat — genOway‑developed, with Cre cassette inserted into rat Prkcd locus
Neural circuit mapping, amygdala / PKCδ neuron function, behavior modulation, optogenetics, feeding and aversion neuroscience
CRISPR/Cas9 targeting, insertion of IRES‑Cre cassette downstream of STOP codon, homologous arms, pronuclear injection, genotyping by PCR and sequencing, anatomical and electrophysiological validation, behavioral assays
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