This study compares human and mouse CD4-targeting minibodies for PET imaging of CD4⁺ T cells. Using the humanized hCD4 Knockin mouse model, researchers demonstrated that a human CD4-specific minibody provides high-contrast, specific imaging of CD4⁺ T cells and can be used to monitor and predict response to immunotherapy. The model allowed precise in vivo evaluation of tracer specificity and translational potential for human use.
The human CD4 minibody demonstrated specific binding and imaging in vivo, and PET imaging using this tracer enabled prediction of cancer immunotherapy response, supporting its use as a noninvasive biomarker tool.
Humanized CD4 (hCD4) Knockin mouse model developed by genOway, with the extracellular domain of human CD4 replacing the murine counterpart, enabling evaluation of human-targeted tracers.
CD4⁺ T cell tracking, Cancer immunotherapy, PET imaging, Noninvasive biomarker, Immunomonitoring
Humanized Knockin model, CD4 extracellular domain replacement, ImmunoPET, Tracer validation, Biomarker development
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
