Overexpression of wild type RRAS2, without oncogenic mutations, drives chronic lymphocytic leukemia

Alejandro M Hortal
Universidad Autónoma de Madrid
January 1, 2022
Mol Cancer
https://pubmed.ncbi.nlm.nih.gov/35120522

This article is currently being updated. View its version on PubMed.

https://pubmed.ncbi.nlm.nih.gov/35120522

Research summary

This study investigates the role of wild-type RRAS2 overexpression in the development of chronic lymphocytic leukemia (CLL). Researchers generated a conditional Knockin mouse model with human RRAS2 inserted into the Rosa26 locus, controlled by a loxP-flanked STOP cassette and an IRES-EGFP reporter. Upon Cre-mediated recombination, RRAS2 is overexpressed in B cells, leading to the development of CLL characterized by CD5+IgM+ B cell expansion, splenomegaly, and reduced survival. The model mirrors human CLL features and is valuable for preclinical testing of therapies.

Key outcome of the study

Overexpression of wild-type RRAS2 in B cells induces CLL-like disease in mice, demonstrating its oncogenic potential without activating mutations and providing a model for therapeutic research.

Model

Conditional Knockin mouse model with human RRAS2 inserted into the Rosa26 locus, featuring a loxP-flanked STOP cassette and an IRES-EGFP reporter; Cre recombinase under the mb1 promoter ensures B cell-specific expression.

TARGET:
RRAS2
TC21

Keywords

Chronic lymphocytic leukemia, B cell malignancy, Oncogene overexpression, Preclinical model

Technical specifications

Knockin model, Rosa26 locus, loxP-flanked STOP cassette, IRES-EGFP reporter, B cell-specific Cre expression

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