Customized Rat Models
Thanks to breakthroughs in genome editing technologies, genetically modified rat models are now widely reconsidered for target validation, and the study and modeling of human diseases.
Customized rat models are ideal tools for identifying new lead compounds, and testing the pharmacodynamics and toxicity of potential therapeutics, the reason why they are widely used in medical research fields, including, but not limited to:
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A pioneer in rat cloning with a long-term history in genetically engineered rat model creation, genOway offers fully integrated and cost-effective services suitable to academic institutions, biotech and pharma companies.
Our customized rat model services
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Constitutive Knockout Permanent inactivation of the target gene, and total absence of the protein in the whole animal, in every cell of the organism |
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| Conditional KO | |
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Tissue-specific Knockout Gene of interest is inactivated in specific cell types in a certain tissue |
Inducible Knockout Gene of interest can be temporally controlled at a given time-point in embryonic, post-natal or adult animals |
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Point mutation Knockin One or more mutated nucleotides causing gain or loss of function of a given protein |
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Quick Knockin Controlled gene expression/copy number by targeting the permissive (neutral) locus Rosa26 |
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We ensure reliable and reproducible performance in many rat strains, including, but not limited to:
- Inbred: Brown Norway, Fischer 344, Lewis, etc.
- Outbred: Wistar, Long-Evans, Sprague Dawley, etc.
Note: we are running, in collaboration with customers, several R&D programs to develop more challenging rat models. If you are interested in developing innovative rat models through partnerships, please reach out to us.
Learn more about our rat models:
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Remember Ralph?Since then, we are heavily investing in research collaboration aiming at improving the creation of genetically modified rat models. |
Important milestones in our rat history:
| 2018 | Generation of immunodeficient rats with Rag1 and Il2rg gene deletions and human tissue grafting models [Ménoret et al., 2018] |
| 2016 | Targeted sequence insertion in rats with CRISPR/Cas9 and TALE nucleases [Renaud et al., 2016] |
| 2014–2016 |
Routine generation of Knockout and point mutation rat models using the CRISPR/Cas9 system Numerous R&D projects for generating Knockin rat models |
| 2015 | Gene targeting in rats using Cas9 and TALEN engineered proteins [Ménoret et al., 2015] |
| 2014 | Gene targeting in rats using TALE nucleases [Remy et al., 2014] |
| 2013 | Immunodeficient rats engineered by meganucleases [Menoret et al., 2013] |
| 2010 | Two book chapters in Rat Genomics on practical guidelines for RNAi working in rats [Petit et al., 2010], and on somatic cell nuclear transfer in rats [Cozzi et al., 2010] |
| 2006–2010 | SME member and reference partner for rat ES cell line evaluation of the Sixth European Framework Program EURATools (European Rat Tools for Functional Genomics), exploring the genetic basis of rat disease characteristics and improving rat models for study of common human diseases |
| 2009 | Book chapter in Transgenesis Techniques on pronuclear DNA injection in rats [Cozzi et al., 2009] |
| 2008 | Review on “Use of genetically modified rat models for translational medicine” [Cozzi J et al., 2008] |
| 2005–2008 | Leading member of the ActivRat R&D project aiming at improving the efficacy of the cloning procedure in rats |
| 2007 | Provider of rat models for human diseases (ENU technology–chemically induced mutagenesis), in collaboration with the Hubrecht Institute, The Netherlands |
| 2005 | Review on “Transgenic modifications of the rat genome” [Tesson et al., 2005] |
| 2003 | Generation of the world’s first cloned rat, in partnership with the National Institute of Agronomic INRA [Zhou et al., Science, 2003] |
| 2002 | Initiation of genetic rat services, in collaboration with the French Institute of Health and Medical Research INSERM |
Selection of genOway clients' publications on rat models
Comasco E, Schijven D, de Maeyer H, Vrettou M, Nylander I, Sundström-Poromaa I, Olivier JD.
Constitutive serotonin transporter reduction resembles maternal separation with regard to stress-related gene expression.
ACS Chem Neurosci. 2019
El-Ayache N, Galligan JJ.
5-HT3 receptor signaling in serotonin transporter knockout rats: a female sex specific animal model of visceral hypersensitivity.
Am J Physiol Gastrointest Liver Physiol. 2018
Micheli L, Di Cesare Mannelli L, Guerrini R, Trapella C, Zanardelli M, Ciccocioppo R, Rizzi A, Ghelardini C, Calò G.
Acute and subchronic antinociceptive effects of nociceptin/orphanin FQ receptor agonists infused by intrathecal route in rats.
Eur J Pharmacol. 2015
Galligan JJ, Patel BA, Schneider SP, Wang H, Zhao H, Novotny M, Bian X, Kabeer R, Fried D, Swain GM.
Visceral hypersensitivity in female but not in male serotonin transporter knockout rats.
Neurogastroenterol Motil. 2013
Lizarraga LE, Phan AV, Cholanians AB, Herndon JM, Lau SS, Monks TJ
Serotonin reuptake transporter deficiency modulates the acute thermoregulatory and locomotor activity response to 3,4-(±)-methylenedioxymethamphetamine, and attenuates depletions in serotonin levels in SERT-KO rats.
Toxicol Sci. 2014
Ali SA, Hanks JE, Stebbins AW, Cohen ST, Hunter DA, Snyder-Warwick AK, Mackinnon SE, Kupfer RA, Hogikyan ND, Feldman EL, Brenner MJ.
Comparison of Myelin-Associated Glycoprotein With Vincristine for Facial Nerve Inhibition After Bilateral Axotomy in a Transgenic Thy1-Gfp Rat Model.
JAMA Facial Plast Surg. 2019
Saheb-Al-Zamani M, Yan Y, Farber SJ, Hunter DA, Newton P, Wood MD, Stewart SA, Johnson PJ, Mackinnon SE.
Limited regeneration in long acellular nerve allografts is associated with increased Schwann cell senescence.
Exp Neurol. 2013
Sun HH, Saheb-Al-Zamani M, Yan Y, Hunter DA, Mackinnon SE, Johnson PJ.
Geldanamycin accelerated peripheral nerve regeneration in comparison to FK-506 in vivo.
Neuroscience. 2012
Moore AM, Borschel GH, Santosa KA, Flagg ER, Tong AY, Kasukurthi R, Newton P, Yan Y, Hunter DA, Johnson PJ, Mackinnon SE.
A transgenic rat expressing green fluorescent protein (GFP) in peripheral nerves provides a new hindlimb model for the study of nerve injury and regeneration.
J Neurosci Methods. 2012
Magill CK, Moore AM, Borschel GH, Mackinnon SE.
A new model for facial nerve research: the novel transgenic Thy1-GFP rat.
Arch Facial Plast Surg. 2010
Ménoret S, Ouisse LH, Tesson L, Delbos F, Garnier D, Remy S, Usal C, Concordet JP, Giovannangeli C, Chenouard V, Brusselle L, Merieau E, Nerrière-Daguin V, Duteille F, Bellier-Waast F, Fraichard A, Nguyen TH, Anegon I.
Generation of immunodeficient rats with Rag1 and Il2rg gene deletions and human tissue grafting models.
Transplantation. 2018
Ménoret S, Fontanière S, Jantz D, Tesson L, Thinard R, Rémy S, Usal C, Ouisse LH, Fraichard A, Anegon I.
Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases.
FASEB J. 2013
Pavlovic D, Hall AR, Kennington EJ, Aughton K, Boguslavskyii A, Fuller W, Despa S, Bers DM, Shattock MJ.
Nitric oxide regulates cardiac intracellular Na+ and Ca2+ by modulating Na/K ATPase via PKCε and phospholemman-dependent mechanism.
J Mol Cell Cardiol. 2013
Franquesa M, Herrero E, Torras J, Ripoll E, Flaquer M, Gomà M, Lloberas N, Anegon I, Cruzado JM, Grinyó JM, Herrero-Fresneda I.
Mesenchymal Stem Cell Therapy Prevents Interstitial Fibrosis and Tubular Atrophy in a Rat Kidney Allograft Model.
Stem Cells Dev. 2012
Lelan F, Boyer C, Thinard R, Rémy S, Usal C, Tesson L, Anegon I, Neveu I, Damier P, Naveilhan P, Lescaudron L.
Effects of Human Alpha-Synuclein A53T-A30P Mutations on SVZ and Local Olfactory Bulb Cell Proliferation in a Transgenic Rat Model of Parkinson Disease.
Parkinsons Dis. 2011
Patrick Davis R, Linder AE, Watts SW.
Lack of the serotonin transporter (SERT) reduces the ability of 5-hydroxytryptamine to lower blood pressure.
Naunyn Schmiedebergs Arch Pharmacol. 2011
Linder AE, Davis RP, Burnett R, Watts SW.
Comparison of the function of the serotonin transporter in the vasculature of male and female rats.
Clin Exp Pharmacol Physiol. 2011
Ren W, Watts SW, Fanburg BL.
Serotonin transporter interacts with the PDGFβ receptor in PDGF-BB-induced signaling and mitogenesis in pulmonary artery smooth muscle cells.
Am J Physiol Lung Cell Mol Physiol. 2011
Johnson PL, Molosh AI, Federici LM, Bernabe C, Haggerty D, Fitz SD, Nalivaiko E, Truitt W, Shekhar A.
Assessment of fear and anxiety associated behaviors, physiology and neural circuits in rats with reduced serotonin transporter (SERT) levels.
Transl Psychiatry. 2019
Rizzi A, Molinari S, Marti M, Marzola G, Calo' G.
Nociceptin/orphanin FQ receptor knockout rats: in vitro and in vivo studies.
Neuropharmacology. 2011
Rutten K, De Vry J, Bruckmann W, Tzschentke TM.
Pharmacological blockade or genetic knockout of the NOP receptor potentiates the rewarding effect of mrp in rats.
Drug Alcohol Depend. 2011
Linder AE, Beggs KM, Burnett RJ, Watts SW.
Body distribution of infused serotonin in rats.
Clin Exp Pharmacol Physiol. 2009
genOway's rat publications
Ménoret S, Ouisse LH, Tesson L, Delbos F, Garnier D, Remy S, Usal C, Concordet JP, Giovannangeli C, Chenouard V, Brusselle L, Merieau E, Nerrière-Daguin V, Duteille F, Bellier-Waast F, Fraichard A, Nguyen TH, Anegon I.
Generation of immunodeficient rats with Rag1 and Il2rg gene deletions and human tissue grafting models.
Transplantation. 2018
Renaud JB, Boix C, Charpentier M, De Cian A, Cochennec J, Duvernois-Berthet E, Perrouault L, Tesson L, Edouard J, Thinard R, Cherifi Y, Menoret S, Fontanière S, de Crozé N, Fraichard A, Sohm F, Anegon I, Concordet JP, Giovannangeli C.
Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases.
Cell Rep. 2016
Ménoret S, De Cian A, Tesson L, Remy S, Usal C, Boulé JB, Boix C, Fontanière S, Crénéguy A, Nguyen TH, Brusselle L, Thinard R, Gauguier D, Concordet JP, Cherifi Y, Fraichard A, Giovannangeli C, Anegon I.
Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins.
Sci Rep. 2015
Remy S, Tesson L, Menoret S, Usal C, De Cian A, Thepenier V, Thinard R, Baron D, Charpentier M, Renaud JB, Buelow R, Cost GJ, Giovannangeli C, Fraichard A, Concordet JP, Anegon I.
Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases.
Genome Res. 2014
Ménoret S, Fontanière S, Jantz D, Tesson L, Thinard R, Rémy S, Usal C, Ouisse LH, Fraichard A, Anegon I.
Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases.
FASEB J. 2013
Book chapter: Cozzi J, Wang E, Jacquet C, Fraichard A, Cherifi Y, Zhou Q.
Procedures for somatic cell nuclear transfer in the rat.
Rat Genomics: Methods Mol Biol. 2010
Book chapter: Petit S, Thiam K.
Interference RNA for in vivo Knock-down of gene expression or genome-wide screening using shRNA.
Rat Genomics: Methods Mol Biol. 2010
Book chapter: Cozzi J, Anegon I, Braun V, Gross AC, Merrouche C, Cherifi Y.
Pronuclear DNA injection for the production of transgenic rats.
Transgenesis Techniques: Methods Mol Biol. 2009
Cozzi J, Fraichard A, Thiam K.
Use of genetically modified rat models for translational medicine.
Drug Discov Today 2008
Tesson L, Cozzi J, Menoret S, Remy S, Usal C, Fraichard A, Anegon I.
Transgenic modifications of the rat genome.
Transgenic Res. 2005
Zhou Q, Renard JP, Le Friec G, Brochard V, Beaujean N, Cherifi Y, Fraichard A, Cozzi J.
Generation of fertile cloned rats by regulating oocyte activation.
Science. 2003
Further reading
- The Importance of Genetic Design When Choosing Your Preclinical Model: Focus on Preclinical Models for Cardiovascular Disorders — A Case Study on BMPR2
- When Rat Models Could Provide Optimized Translatability: The Case of CD89
- Back to the rat
Follow this link if you seek another transgenic model.