Consolidation of cell‑ECM adhesion through direct talin‑mediated actin linkage is essential for mouse embryonic morphogenesis

Deng W
University of British Columbia
June 21, 2025
Commun Biol
https://pubmed.ncbi.nlm.nih.gov/40544224/

This article is currently being updated. View its version on PubMed.

https://pubmed.ncbi.nlm.nih.gov/40544224/

Research summary

This study shows that disrupting the talin‑1 actin‑binding site (ABS3) via the KVK/DDD point mutation impairs focal adhesion maturation, cell migration, and leads to embryonic lethality in mice.

Key outcome of the study

ABS3 mutation causes embryonic lethality, disrupted actin organization, and defective cell–ECM adhesion, establishing the critical role of talin-mediated actin binding in morphogenesis.

Model

Tln1^KVK/DDD point mutation Knockin mouse model developed by genOway on C57BL/6 background, allowing assessment of talin‑actin linkage during embryogenesis and in primary fibroblasts.

TARGET:
Tln1
Talin‑1

Keywords

Developmental biology, Cell adhesion, Mechanobiology, Embryogenesis

Technical specifications

Knockin, Point mutation (KVK>DDD), Actin-binding disruption, C57BL/6

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