This study presents a protocol for assessing central auditory processing in awake mice. The researchers implanted electrodes above the left auditory cortex (AC) and the left inferior colliculus (IC), along with a headpost for stabilization. This setup allowed for the recording of auditory brainstem responses (ABRs) and auditory steady-state responses (ASSRs) to various auditory stimuli, enabling the evaluation of auditory processing at both cortical and subcortical levels.
The Shank3 mutant mice exhibited degraded phase-locking at high sound repetition rates (above 60 Hz) in the AC, with similar alterations observed in the IC. These findings suggest that mutations in Shank3 may affect fast temporal processing in multiple auditory regions, providing insights into the neural mechanisms underlying auditory processing disorders.
The study utilized a mouse model with a deletion in the Shank3 gene (Shank3∆11/∆11), which is associated with autism spectrum disorders and known to affect synaptic function. This model was used to investigate alterations in central auditory processing.
Central auditory processing disorders, Autism spectrum disorders, Auditory neuroscience, Temporal processing, Synaptic function
Electrode implantation, Head-fixation, Auditory brainstem response (ABR), Auditory steady-state response (ASSR), In vivo electrophysiology
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