This study investigates the role of transzonal projections (TZPs), filopodia-like structures from somatic follicular cells, in oocyte development. Using Myo10<sup>−/−</sup> mice, researchers demonstrate that the absence of MYO10 reduces TZP density, leading to impaired oocyte–matrix integrity, altered gene expression, and decreased developmental competence, ultimately reducing fertility.
Loss of MYO10 leads to decreased TZP density, affecting oocyte–somatic cell communication, altering oocyte morphology and transcriptome, and reducing fertility.
Myo10(−/−) (myosin-X Knockout) mouse model developed by genOway, featuring a neomycin selection cassette inserted between exons 22 and 23 of the Myo10 gene, disrupting MYO10 expression in all cell types.
Oocyte development, Fertility, Cell–cell communication, Transzonal projections, Reproductive biology
Constitutive Knockout model, Neomycin selection cassette insertion, Disruption of MYO10 expression, Analysis of oocyte morphology and gene expression
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
