GalNT2-mediated O-glycosylation affects pancreas development and function in mice

Mercanoglu B
November 30, 2024
Sci Rep
https://pubmed.ncbi.nlm.nih.gov/39613794

This article is currently being updated. View its version on PubMed.

https://pubmed.ncbi.nlm.nih.gov/39613794

Research summary

This study investigates the impact of GalNT2 overexpression on pancreatic development and function. Conditional overexpression of GalNT2 in the pancreas causes acinar cell loss, pancreatic steatosis, and in homozygous mice, complete pancreatic loss and lethality. Proteomic analyses identified new O-glycosylation targets and support a role for GalNT2 in cellular transdifferentiation.

Key outcome of the study

GalNT2 overexpression in the pancreas leads to loss of acinar cells, pancreatic steatosis, lethality in homozygous mice, and transdifferentiation into adipocytes. Proteomic analysis revealed additional O-glycosylation targets contributing to these phenotypes.

Mouse model

Conditional Galnt2 overexpression mouse model developed by genOway, with the Galnt2 transgene inserted into the Rosa26 locus. The model features a floxed STOP cassette allowing Cre-dependent overexpression, and was crossed with Ptf1a-Cre for pancreas-specific activation.

TARGET:
Galnt2
Polypeptide N-acetylgalactosaminyltransferase 2

Keywords

Pancreas development, O-glycosylation, Metabolic dysfunction, Cell fate reprogramming, Glycobiology

Technical specifications

Conditional overexpression model, Rosa26 locus targeting, Floxed STOP cassette, Ptf1a-Cre driver, Tissue-specific activation, O-glycoproteomics

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