This study investigates the role of Sca-1⁺ cells in vein graft remodeling. Utilizing single-cell RNA sequencing and inducible lineage-tracing mouse models, the research identifies Sca-1⁺ cells from various sources contributing to endothelial and smooth muscle cell formation in vein grafts.
Sca-1⁺ cells from recipient arteries contribute to endothelial regeneration at perianastomotic regions, while donor vein-derived Sca-1⁺ cells differentiate into smooth muscle cells in the neointima. Bone marrow-derived Sca-1⁺ cells primarily become inflammatory cells.
Sca-1 (Ly6a)-CreERT2; Rosa26-tdTomato lineage-tracing mouse model, enabling inducible labeling of Sca-1⁺ cells to trace their fate during vein graft remodeling.
Vascular biology, Vein graft remodeling, Endothelial regeneration, Smooth muscle cell differentiation, Lineage tracing
Inducible Cre-loxP system, Rosa26-tdTomato reporter, Sca-1 (Ly6a) promoter-driven CreERT2, Lineage tracing, Vein graft transplantation model
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
