This study investigates the role of NEDD9 overexpression in mammary gland development and its cooperation with the HER2 oncogene in tumor initiation. The researchers generated a conditional transgenic mouse model with NEDD9 overexpression targeted to the Rosa26 locus. When crossed with MMTV-Cre mice, NEDD9 overexpression led to mammary epithelial hyperplasia, increased branching, and expansion of luminal epithelial cells. Co-expression with the Erbb2 (neu) oncogene accelerated the development of preneoplastic lesions.
NEDD9 overexpression in the mammary gland induces hyperplasia and expansion of luminal epithelial cells. When combined with Erbb2 overexpression, it accelerates tumor initiation, suggesting that NEDD9 acts as a facilitator of HER2-driven tumorigenesis and may serve as a prognostic marker in breast cancer.
NEDD9 conditional overexpression mouse model developed by genOway, featuring a cDNA sequence for human NEDD9 under the control of the CAG promoter, inserted into the Rosa26 locus with a floxed STOP cassette, allowing for tissue-specific expression upon Cre recombination.
Breast cancer, HER2-positive tumors, Mammary gland development, Tumor initiation, Prognostic biomarkers
Conditional overexpression model, Rosa26 locus targeting, CAG promoter, Floxed STOP cassette, MMTV-Cre driver, HER2/Erbb2 cooperation
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
