This study investigates the role of neuromedin U (NMU) neurons in the Edinger–Westphal (EW) nucleus in response to alcohol exposure. The researchers aimed to determine whether NMU neurons are activated by alcohol and how this activation relates to urocortin 1 (UCN1) neurons, which are known to be involved in alcohol consumption behaviors.
The study found that NMU neurons in the EW nucleus are responsive to alcohol exposure. Importantly, this activation does not interfere with the activity of UCN1 neurons in the same region, suggesting that NMU and UCN1 neurons have distinct roles in the brain's response to alcohol.
The study utilized a Knockin mouse model developed in collaboration with genOway. This Nmu-Cre Knockin mouse was engineered to express Cre recombinase under the control of the endogenous Nmu promoter, allowing for specific targeting of NMU-expressing neurons. The design includes an internal ribosome entry site (IRES) to ensure bicistronic expression of Cre recombinase and NMU, preserving the native expression pattern of NMU. This configuration enables the use of Cre-dependent reporter systems, such as fluorescent proteins, to visualize and study NMU neurons' activity and connectivity. By crossing the Nmu-Cre mice with reporter lines, the study achieved specific labeling of NMU neurons, facilitating detailed analysis of their response to alcohol exposure.
Neuroscience, Alcohol-related behaviors, Neuropharmacology, Addiction research, Behavioral neuroscience
Knockin, Cre recombinase, Internal ribosome entry site (IRES), Cre-dependent reporter system, Fluorescent protein labeling, Neuronal activity mapping
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
