This study investigates how aging affects the fidelity of protein synthesis across different organs in mice. Researchers utilized a Knockin mouse model with a fluorescent and luminescent read-out system to assess translation fidelity over time.
The findings reveal that translational errors increase with age in an organ-dependent manner, with significant increases observed in muscle and brain tissues, while the liver maintained translation fidelity. This suggests that certain organs are more susceptible to age-related declines in protein synthesis accuracy.
Rosa26 Knockin reporter mouse model developed by genOway, in which the tdKat2-TGA(Stop)-firefly luciferase construct was inserted into the Rosa26 locus. This system allows for detection of translational errors via fluorescence (tdKat2) and bioluminescence (luciferase) in an organ-dependent manner.
Aging, Protein synthesis fidelity, Organ-specific aging, Ribosomal function
Knockin mouse model, Rosa26 locus, Translation fidelity reporter, tdKat2-TGA(Stop)-luciferase, Fluorescence & bioluminescence read-out
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