Labeling for Cell Trafficking
This Knockin technology targets the insertion of a reporter in any cell-specific gene enabling a tight regulation of the reporter expression and consequently, a reliable cell labeling.
Genetically modified models have been developed to:
- Label one-cell lineage using one biomarker specific to the cell lineage of interest.
- Label one subpopulation of one-cell lineage, i.e. activated cells, as defined by the expression of a specific biomarker.
Case study 1: A Sox9-eGFP Knockin model enables to follow stage-specific changes of the Sertoli-germ cell mass in developing testis.
Adapted from Nel-Themaat et al. Dev Dyn 2009. Morphometric analysis of testis cord formation in Sox9-EGFP mice.
Labelled Cells Enable Trafficking Studies
Between E11.5 and E12.5, the testis increase in thickness and the first signs of differentiation appeare.
Formation of small protrusions of the Sertoli-germ cell mass.
Distinct, non-fluorescing areas in the tissue mass.