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Quick KI cells
Cell line generation

Quick KI cells

A quick Knockin cell line has a transgene inserted into permissive loci (e.g., Rosa26). The transgene overexpression is constitutively or inducibly.

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A Quick Knockin cell line defines a model in which a transgene is inserted in a permissive locus, also known as neutral locus or safe harbor, such as Rosa26. The transgene can be constitutively or inducibly overexpressed.

Targeting those loci enables full control of the copy number and avoids transgene silencing or deregulation of neighbouring genes.

Applications

For academic research:

  • Gene function studies
  • Study disease-causing transgene, mutated or not

For bio-pharmaceutical research & development:

  • Compound screening
  • Selectivity screening
  • Drug affinity testing
  • Compound profiling

Strengths of Quick Knockin cell models

  • Stable and predictable expression
  • Almost no position effect variegation
  • Almost no transgene silencing
  • No deregulation of neighbouring gene

Limitations of Quick Knockin cell models

  • Exogenous regulation may result in lower physiological relevancy
  • Limited physiological relevancy when multiple isoforms have been described
  • Problem of stoichiometry if interaction with other partners for signaling
    → Must be solved by an in-depth scientific analysis and a rationale risk assessment prior to model development, all included in our project feasibility assessment
  • Not functional for expression of non-dominant mutations
    → Choose alternative approaches such as, e.g., point mutation Knockin

Validation

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Quick KI cells

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Reporter KI cells

A reporter Knockin cell line is a useful model when there are no available antibodies for the target or when protein measurement is laborious and costly.

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Point mutation KI cells

A point mutation Knockin cell line has one or more mutated nucleotides that alter the protein amino acid sequence, changing its functions or properties.

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Constitutive KO cells

A constitutive Knockout cell line refers to a conventional KO; a model in which the target gene is permanently inactivated, and no protein is produced.

Discover our off-the-shelf models.

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Quick Knockin cell lines (Neutral loci targeting such as Rosa26)

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