A humanized β 2 integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in vivo

Lai Wen
La Jolla Institute for Immunology
January 1, 2022
Cell Rep
https://pubmed.ncbi.nlm.nih.gov/35649374

This article is currently being updated. View its version on PubMed.

https://pubmed.ncbi.nlm.nih.gov/35649374

Research summary

This study presents the development of a humanized β₂ integrin Knockin mouse model, where the human ITGB2 gene replaces the mouse Itgb2 gene. This model enables in vivo visualization of β₂ integrin activation using conformation-specific antibodies (KIM127 and mAb24). Utilizing this model, researchers observed localized β₂ integrin activation at the rear of arresting neutrophils in inflamed venules and during systemic inflammation, providing insights into neutrophil adhesion dynamics and integrin activation patterns in vivo.

Key outcome of the study

The humanized ITGB2 Knockin mouse model enables real-time visualization of β₂ integrin activation in neutrophils during inflammation, revealing spatially localized activation patterns critical for neutrophil adhesion and migration.

Mouse model

Humanized ITGB2 Knockin mouse model with the human ITGB2 gene replacing the mouse Itgb2 gene, allowing in vivo detection of β₂ integrin activation using specific antibodies.

TARGET:
ITGB2
CD18, Integrin beta-2

Keywords

Neutrophil adhesion, Integrin activation, Inflammation, Humanized mouse model

Technical specifications

Knockin model, Human gene replacement, In vivo imaging, Conformation-specific antibody detection

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