This study presents the development of a humanized β₂ integrin Knockin mouse model, where the human ITGB2 gene replaces the mouse Itgb2 gene. This model enables in vivo visualization of β₂ integrin activation using conformation-specific antibodies (KIM127 and mAb24). Utilizing this model, researchers observed localized β₂ integrin activation at the rear of arresting neutrophils in inflamed venules and during systemic inflammation, providing insights into neutrophil adhesion dynamics and integrin activation patterns in vivo.
The humanized ITGB2 Knockin mouse model enables real-time visualization of β₂ integrin activation in neutrophils during inflammation, revealing spatially localized activation patterns critical for neutrophil adhesion and migration.
Humanized ITGB2 Knockin mouse model with the human ITGB2 gene replacing the mouse Itgb2 gene, allowing in vivo detection of β₂ integrin activation using specific antibodies.
Neutrophil adhesion, Integrin activation, Inflammation, Humanized mouse model
Knockin model, Human gene replacement, In vivo imaging, Conformation-specific antibody detection
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
