An adherent-invasive Escherichia coli-colonized mouse model to evaluate microbiota-targeting strategies in Crohn’s disease

Adeline Sivignon
Université Clermont Auvergne
November 1, 2022
Dis Model Mech
https://pubmed.ncbi.nlm.nih.gov/36172858

This article is currently being updated. View its version on PubMed.

https://pubmed.ncbi.nlm.nih.gov/36172858

Research summary

This study describes the development of a transgenic mouse model expressing human CEACAM6 in the small intestine, allowing for colonization by adherent-invasive Escherichia coli (AIEC), a pathobiont linked to Crohn’s disease. The model serves as a platform to study host-pathogen interactions and evaluate microbiota-targeting therapies.

Key outcome of the study

Expression of human CEACAM6 in the ileum enables enhanced colonization by AIEC, providing a physiologically relevant in vivo model for testing anti-AIEC strategies in Crohn’s disease.

Model

Vill-hCC6 Knockin mouse model developed by genOway, expressing human CEACAM6 under the villin promoter. The transgene was inserted into the murine Hprt locus using homologous recombination in 129P2/OlaHsd ES cells.

TARGET:
CEACAM6
CD66c, NCA, Carcinoembryonic antigen-related cell adhesion molecule 6

Keywords

Crohn’s disease, AIEC infection, Intestinal inflammation, Host-pathogen interaction, Microbiota therapeutics

Technical specifications

Knockin model, Hprt locus targeting, Villin promoter, Human CEACAM6 expression, AIEC colonization

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