Using human lung biopsies as reference, the study assessed lung immune cell composition in humanized BRGSF‑HIS mice engrafted with human CD34⁺ hematopoietic stem cells. They found human NK cells, CD4⁺ and CD8⁺ T cells, γδ T cells, regulatory T cells, monocytes and dendritic cells in the lung interstitium at frequencies comparable to human lungs. Mouse‑origin alveolar macrophages dominated alveoli. Exogenous human G‑CSF promoted egress of human CD15⁺ neutrophils. Human T cells responded to CD3 stimulation and myeloid cells secreted human cytokines (IL‑6, CCL17, IL‑10, IL‑1RA) upon LPS challenge.
Humanized BRGSF mice display functional human lymphoid and myeloid lung‑resident cells in interstitium; human neutrophil egress inducible by hG‑CSF, human T cells and myeloid cells from lung are functionally responsive in vitro and in vivo
genO-BRGSF-HIS humanized mouse, reconstituted with human CD34⁺ HSCs — genOway-developed
Lung immune modeling, humanized mice, lung interstitial immunity, human CD34⁺ engraftment, translational immunology
CD34⁺ human HSC engraftment into BRGSF mice, lung tissue immunophenotyping, neutrophil mobilization via hG‑CSF, T cell stimulation assays, myeloid cytokine secretion assays
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